Anabolic response to acute resistance exercise and protein ingestion in young men Not Applicable
Conditions
Interventions
This study will employ a within-subjects repeated measures design, comprising of ten resistance-trained male participants. After consenting to participate, participants will be asked to attend the lab
bioelectrical spectroscopy) assessed and provide baseline breath, blood and urine samples. Participants will then complete a 35 min whole-body resistance exercise training session using machine weight
Sponsors
Dublin City University
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Healthy males aged between 18 and 40 years of age 2. Non-smokers 3. Free from chronic metabolic disease and coagulation disorders 4. Not have any chronic medication use for >6 months 5. Body mass index of 2 years or two or more resistance exercise training sessions per week
Exclusion criteria
Exclusion criteria: 1. 40 years of age 2. Smokers 3. Suffering or previously suffering from a chronic metabolic disease and/or coagulation disorder 4. Chronic medication use >6 months 5. Body mass index >30 kg/m² 6. Currently of previously used performance enhancing drugs 7. Resistance training history <2 years
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Dietary net leucine balance (the difference between leucine intake and total exogenous leucine oxidation), measured by ¹³CO2 enrichment of breath samples by isotope-ratio mass spectrometry over the 6-hour recovery period | — |
Secondary
| Measure | Time frame |
|---|---|
| Between-condition comparison of: 1. Total amino acid concentrations in plasma measured by high-performance liquid chromatography at 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, 210, 240, 270, 300, 330, and 360 minutes after ingestion of the drinks. Area under the curve (AUC), peak concentration (Cmax), and time to peak concentration (Tmax) will be calculated from these data points 2. Essential amino acid concentrations in plasma measured by high-performance liquid chromatography at 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, 210, 240, 270, 300, 330, and 360 minutes after ingestion of the drinks. AUC, Cmax, and Tmax will be calculated from these data points 3. Branched-chain amino acid concentrations in plasma measured by high-performance liquid chromatography at 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, 210, 240, 270, 300, 330, and 360 minutes after ingestion of the drinks. AUC, Cmax, and Tmax will be calculated from these data points 4. Non-essential amino acid concentrations in plasma measured by high-performance liquid chromatography at 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, 210, 240, 270, 300, 330, and 360 minutes after ingestion of the drinks. AUC, Cmax, and Tmax will be calculated from these data points 5. Individual amino acid concentrations in plasma measured by high-performance liquid chromatography at 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, 210, 240, 270, 300, 330, and 360 minutes after ingestion of the drinks. AUC, Cmax, and Tmax will be calculated from these data points 6. Alpha-ketoisocaproate (KIC) concentrations in plasma measured by high-performance liquid chromatography at 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, 210, 240, 270, 300, 330, and 360 minutes after ingestion of the drinks. AUC, Cmax, and Tmax will be calculated from these data points 7. [¹³C]leucine in urine measured by liquid chromatography tandem mass spectrometry (LC/MS/MS) before exercise, and in pooled samples collected over 6 hours after ingestion of the drinks 8. Breat | — |
Countries
Ireland
Outcome results
None listed