Periodontitis Oral Health Periodontal disease, unspecified
Conditions
Interventions
Venous blood and oral rinse samples were obtained from 19 patients with untreated periodontitis and 16 control subjects without periodontitis for PMN isolation. Cell numbers were counted and apoptosis
Sponsors
University of Amsterdam
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Patients with untreated periodontitis 2. The presence of bone loss on = 1/3 of the root length on minimum 2 non-adjacent teeth on vertical bite-wing radiographs not older than 12 months 3. Aged 18 and over
Exclusion criteria
Exclusion criteria: 1. A history of pathologic conditions that are known to systemically affect PMN functionality (such as hematological disorders, diabetes mellitus, antibiotics use within the last 6 months, recent history of illness or fever, allergies, alcoholism and pregnancy) 2. Less than 20 teeth 3. Removable partial dentures 4. Night guards 5. Orthodontic banding 6. (Peri) oral piercings 7. Apparent oral lesions
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The presence of PMNs in the oral cavity of periodontitis patients, measured using flow cytometry directly after sampling. All samples were analyzed using flow cytometry on the same day. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Cell counts, measured using a Muse cell counting device 2. Cell membrane integrity, measured with flow cytometric analysis using propidium iodide staining 3. PMN expression markers of cell activation: CD11b, CD63, CD66b and corrected for non-specific binding of isotype control antibodies 4. Presence of constitutive oxygen radicals (ROS), detected using dihydrorhodamine 123 5. Presence of ROS in response to stimulation, determined in samples incubated with phorbol myristate acetate (PMA) or Fusobacterium nucleatum All outcomes measured at a single timepoint | — |
Countries
Netherlands
Outcome results
None listed