Determination of the metabolic response to fructose after fast versus slow ingestion of 100% fruit juice Nutritional, Metabolic, Endocrine
Conditions
Interventions
Thirty healthy, nonobese participants without any systemic disease and receiving no medications and alcohol are included in this study. Each subject is given a total of 500 ml of 100% pure, commercial
Sponsors
Koc University Research Center for Translational Medicine (KUTTAM)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Healthy, nonobese participants 2. No systemic diseases 3. Not taking medications or alcohol
Exclusion criteria
Exclusion criteria: 1. Obesity 2. Any systemic disease 3. Receiving any medications routinely 4. Significant alcohol use
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Serum glucose measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 2. Insulin measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 3. FGF-21 measured using sandwich enzyme-linked immunosorbent assay (ELISA) using commercial kits (Cloud-Clone Corp., Wuhan, China) at baseline, and 15, 30, 60, and 120 min after drinking apple juice. Sera is separated in aliquots and is frozen rapidly at -80 °C for the measurements. 4. Copeptin measured using sandwich enzyme-linked immunosorbent assay (ELISA) using commercial kits (Cloud-Clone Corp., Wuhan, China) at baseline, and 15, 30, 60, and 120 min after drinking apple juice. Sera is separated in aliquots and is frozen rapidly at -80 °C for the measurements. 5. Osmolarity measured using the freezing-point Osmometer K-7400S (Knauer, Berlin, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 6. Sodium measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 7. BUN measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 8. Lactic acid measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 9. Uric acid measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice 10. Phosphate measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) at baseline, and 15, 30, 60, and 120 min after drinking apple juice | — |
Secondary
| Measure | Time frame |
|---|---|
| HOMA-IR measured using the following formula: HOMA-IR ?= ?(plasma glucose? ×? plasma insulin/405), where glucose is in mass units (mg/dL), measured using a Roche Cobas 6000 analyzer (Roche, Mannheim, Germany) and calculated for baseline, and 15, 30, 60, and 120 min after drinking apple juice | — |
Countries
Turkey
Contacts
Public ContactLale Ertuglu
Outcome results
None listed