Intestinal permeability Digestive System
Conditions
Interventions
Each participant is his own control. On the first day participants came in fasted overnight (=resting condition) and ingested a sugar probe solution containing 5 grams lactulose, 1 gram L-rhamnose, an
Sponsors
Wageningen University and Research Centre
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Male cyclists/triathletes with at least two years of cycling experience 2. Cycling at least two times a week during peak season 3. 18-35 years old 4. Body mass index (BMI) 20-25 kg/m2 5. Meeting criteria of valid max-test 6. Suitable veins for blood sampling
Exclusion criteria
Exclusion criteria: 1. Known symptoms of milk allergy 2. Known symptoms of immune disease, such as diabetes, gastritis, and coeliac disease 3. Known symptoms of intestinal disease, such as Crohn’s Disease, ulcerosis, and irritable bowel syndrome 4. Smoking 5. Use of hard drugs 6. Chronic use of NSAIDs: aspirins, ibuprofen, etc. 7. Using medicines for gastric and/or intestinal function 8. Participation in other scientific studies 9. Blood donation during the last six weeks before the start of the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Plasma casein and casein-derived peptide levels with and without exercise-induced permeability of the gastrointestinal tract. Plasma samples are taken at t=0, 15, 30, 60, 90, 120, and 300min after intake of casein directly after the control condition (no exercise) or the 1.5hr cycling test. Levels of casein and casein-derived peptides will be analysed in these plasma samples with ELISA and UPLC-MS/MS. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Intestinal permeability as assessed by multisugar test Subjects will receive a multisugar solution to assess permeability directly after the control condition (no exercise) or the 1.5hr cycling test. Urine will be collected for 5 hours at hourly intervals after intake. Urinary sugar levels will be analyzed with HPLC 2. Health effects of exercise-induced permeability and plasma casein and casein-derived peptides Other parameters: serum parameters of inflammation, exercise metabolism, and gastrointestinal function. | — |
Countries
Netherlands
Contacts
Public ContactLonneke JanssenDuijghuijsen
Outcome results
None listed