The effect of different intensities of exercise on intestinal and immune function in healthy young men in the age of 18-35 years old

The effect of different intensities of exercise on biomarkers of GastRo-INTestinal Activity and immune function in healthy young men

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN13656034

Enrollment

Registered

2017-11-03

Start date

2014-09-01

Completion date

Unknown

Last updated

2023-01-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Gastro-intestinal function and immune response Digestive System Gastro-intestinal function and immune response

Interventions

Fifteen healthy young men (20-35 yrs, VO2 max 56.9 ± 3.9 ml. kg-1 min-1 ) performed in a cross-over design five experimental protocols of one hour with different intensity and/or hydration status: 1)

Eligibility

Sex/Gender

Male

Inclusion criteria

Inclusion criteria: 1. Male 2. Generally healthy 3. Recreational athlete with at least two years of cycling experience of at least twice a week 4. 18-35 years old 5. Meeting criteria of valid max-test 6. Body mass index (BMI) 20-25 kg/m2 7. Veins suitable for blood sampling at inspection

Exclusion criteria

Exclusion criteria: 1. Known symptoms of immune diseases such as diabetes, celiac disease, gastric disease or cystic fibrosis 2. Known symptoms of intestinal diseases such as Crohn’s disease, ulcerosis, irritable bowel syndrome or cystic fibrosis 3. Smoking 4. Use of hard drugs 5. Use of specific medicines: 6. Chronic use of NSAIDs: aspirin, ibuprofen, etc. 7. Drugs for gastric and/or intestinal function 8. Participation in other scientific studies 9. Blood donation during the last six weeks prior to the start of the study

Design outcomes

Primary

Measure	Time frame
1. Intestinal permeability (lactulose/rhamnose ratio) is measured using HPAEC (with pulsed electrochemical detection) at baseline, and each hour after rest and exercise up to 5 hours 2. Plasma levels of glutamine is measured using the UFLC (ultra fast liquid chromatography) at baseline, during (30 min) and after rest and exercise (60, 90, 120, 180, 360 min) up to 24 hours 3. Glutamine-derived amino acids are measured using the UFLC (ultra-fast liquid chromatography) at baseline, during (30 min) and after rest and exercise (60, 90, 120, 180, 360 min) up to 24 hours	—

Measure

Time frame

1. Intestinal permeability (lactulose/rhamnose ratio) is measured using HPAEC (with pulsed electrochemical detection) at baseline, and each hour after rest and exercise up to 5 hours 2. Plasma levels of glutamine is measured using the UFLC (ultra fast liquid chromatography) at baseline, during (30 min) and after rest and exercise (60, 90, 120, 180, 360 min) up to 24 hours 3. Glutamine-derived amino acids are measured using the UFLC (ultra-fast liquid chromatography) at baseline, during (30 min) and after rest and exercise (60, 90, 120, 180, 360 min) up to 24 hours

—

Secondary

Measure	Time frame
Gastro-intestinal integrity and inflammation markers are measured using ELISA at baseline, during (30 min) and after rest and exercise (60, 90, 120, 180, 360 min) up to 24 hours.	—

Countries

Netherlands

Contacts

Public ContactShirley Kartaram

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026