Impact of moderate intensive physical activity on bioavailability of flavanones in sedentary females

The effect of a four week exercise intervention on bioavailability of orange juice flavanones in healthy sedentary females

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN13566025

Enrollment

Registered

2016-08-10

Start date

2014-11-05

Completion date

Unknown

Last updated

2016-10-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Bioavailability of orange juice flavanones Nutritional, Metabolic, Endocrine Bioavailability of orange juice flavanones

Interventions

Participants will conduct two 24 hour orange juice feeding trials, one before and another after 4 weeks of moderate intensity exercise intervention. Exercise intervention will involve endurance exer

Eligibility

Sex/Gender

Female

Inclusion criteria

Inclusion criteria: 1. Sedentary status for at least 6 weeks 2. Healthy 3. BMI over 30 4. Normotensive 5. Non-smoker 6. Not taking any drug therapies 7. Aged 18 years and over

Exclusion criteria

Exclusion criteria: 1. History of gastrointestinal disease 2. Vegetarian 3. Unstable food habits 4. Pregnancy

Design outcomes

Primary

Measure	Time frame
Concentration of naringenin and hesperetin metabolites and phenolic acids in urinary fractions collected overnight (0 hours) and after ingestion of orange juice (0-2, 2-5, 5-8, 8-10, 10-24 hours) is measured before and after 4 week exercise intervention by using HPLC-PDA-MS and GC-MS.	—

Secondary

Measure	Time frame
1. Concentration of naringenin and hesperetin metabolites and phenolic acids in plasma collected overnight (0 hours) and at 1, 2, 3, 4, 5, 6, 7, 8, 24 h after the juice intake is measured before and after 4 week exercise intervention by using HPLC-PDA-MS and GC-MS 2. Flavanones in orange juice (Tropicana, Tesco) used for 24 hour feeding trials are measured using HPLC-PDA-MS 3. Mouth to caecum transit time is breath samples collected in the fasted state (0 hours) and at 30 minute intervals for 8 hours after the juice intake is measured before and after 4 week exercise intervention by using a breath hydrogen monitor 4. Body weight is measured in the fasted state in kilograms before and after 4 week exercise intervention using Tanita TBF-410GS Body Composition Analyzer 5. Body fat percentage is measured in the fasted state before and after 4 week exercise intervention using Tanita TBF-410GS Body Composition Analyzer 6. Waist circumference in centimetres is measured in the fasted state before and after 4 week exercise intervention using a measuring tape for circumferences 7. Blood pressure and heart rate is measured in the fasted state before and after 4 week exercise intervention using Omron 7051T blood pressure monitor 8. Ferric reducing ability of plasma (FRAP) in plasma collected in the fasted state (0minutes) and at 1, 2, 3, 4, 5, 6, 7, 8, 24 hours after the juice intake is measured before and after 4 week exercise intervention by using Automated FRAP assay 9. Plasma biomarkers of inflammation such as C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in plasma collected in the fasted state (0minutes) and at 1, 2, 3, 4, 5, 6, 7, 8, 24 hours after the juice intake is measured before and after 4 week exercise intervention by using commercially available ELISA kits	—

Measure

Time frame

1. Concentration of naringenin and hesperetin metabolites and phenolic acids in plasma collected overnight (0 hours) and at 1, 2, 3, 4, 5, 6, 7, 8, 24 h after the juice intake is measured before and after 4 week exercise intervention by using HPLC-PDA-MS and GC-MS 2. Flavanones in orange juice (Tropicana, Tesco) used for 24 hour feeding trials are measured using HPLC-PDA-MS 3. Mouth to caecum transit time is breath samples collected in the fasted state (0 hours) and at 30 minute intervals for 8 hours after the juice intake is measured before and after 4 week exercise intervention by using a breath hydrogen monitor 4. Body weight is measured in the fasted state in kilograms before and after 4 week exercise intervention using Tanita TBF-410GS Body Composition Analyzer 5. Body fat percentage is measured in the fasted state before and after 4 week exercise intervention using Tanita TBF-410GS Body Composition Analyzer 6. Waist circumference in centimetres is measured in the fasted state before and after 4 week exercise intervention using a measuring tape for circumferences 7. Blood pressure and heart rate is measured in the fasted state before and after 4 week exercise intervention using Omron 7051T blood pressure monitor 8. Ferric reducing ability of plasma (FRAP) in plasma collected in the fasted state (0minutes) and at 1, 2, 3, 4, 5, 6, 7, 8, 24 hours after the juice intake is measured before and after 4 week exercise intervention by using Automated FRAP assay 9. Plasma biomarkers of inflammation such as C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in plasma collected in the fasted state (0minutes) and at 1, 2, 3, 4, 5, 6, 7, 8, 24 hours after the juice intake is measured before and after 4 week exercise intervention by using commercially available ELISA kits

—

Countries

United Kingdom

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026