Nutritional study in healthy individuals Nutritional, Metabolic, Endocrine
Conditions
Interventions
Randomisation is carried out using a Williams Design Latin Square with four trial sequence possibilities. These four sequences are randomized in 2 blocks of 8 and 1 of 4, using an online randomization
SUB = moderate dairy intake (~16 g protein)
HIGH+JUMP = high dairy intake (~26 g protein) + ~5 min jumping exercise.
The duration of the intervention per subject is 4 weeks (i.e. 4 test days separated by 1 week).
Sponsors
HAN University of Applied Sciences
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Males and females 2. Age =20 and =40 years. 3. BMI =18.5 and =27.5 kg/m²
Exclusion criteria
Exclusion criteria: 1. Blood donation during the study period 2. Currently smoking 3. Consumption of >21 alcoholic beverages per week 4. Use of illicit drugs 5. Regular use of protein or calcium supplements 6. A self-reported reported lactose intolerance, allergy or sensitivity to dairy ingredients 7. Reported slimming or medically prescribed diet 8. Use of antibiotics in the past month 9. Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, rheumatoid arthritis, orthopaedic disorders, renal disease, liver disease, diabetes mellitus, inflammatory disease, cognitive impairment, and thyroid or parathyroid disease) 10. Use of medications known to interfere with selected outcome measures (i.e. corticosteroids) 11. (Chronic) injuries of the locomotor system that can interfere with the intervention 12. Current participation in another biomedical research study 13. Trained individuals (i.e. performing sport activities for more than 6 hours per week) 14. Structural or competitively participating in exercise/sports with a substantial high-impact component, such as soccer, volleyball, running, and lower body resistance training
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Postprandial kinetics and bioavailability of amino acids measured using blood samples at baseline, 15, 30, 45, 60, 90, 120, 180, 240, and 300 minutes | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Glycaemic response measured using blood samples (glucose, insulin, GLP-1) at baseline, 15, 30, 45, 60, 90, 120, 180, 240, and 300 minutes 2. Bone metabolism measured using blood samples (calcium, PTH, CTX, P1NP) at baseline, 15, 30, 45, 60, 90, 120, 180, 240, 300 minutes, and 24 hours 3. Feelings of hunger and satiety measured using the visual analogue score (VAS) baseline, 60, 120, 180, 240, and 300 minutes | — |
Countries
Netherlands
Outcome results
None listed