The effect of oral capsinoids supplementation on exercise-induced fatigue and molecular biomarkers involved in skeletal muscle fuel recovery in humans

Fffect of acute post-exercise capsinoids supplementation on energy substrate utilization in young adults

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN13356971

Enrollment

Registered

2019-08-07

Start date

2016-04-15

Completion date

Unknown

Last updated

2019-09-09

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

All participants in this study were non-alcoholics and non-smokers with a stable medical condition. To avoid the influence of other factors on results, participants were instructed to have a balanced diet and healthy lifestyle. In addition, the consumption of caffeine, tea, and tobacco was strictly prohibited during the experimental period. Not Applicable

Interventions

Participants in this crossover designed study were randomly assigned into two trials, including placebo and CSN (capsinoids). All participants performed a 60-min cycling exercise on a

Eligibility

Sex/Gender

Male

Inclusion criteria

Inclusion criteria: 1. Healthy male college student. 2. Non-alcoholic. 3. Non-smoker. 4. Stable medical condition.

Exclusion criteria

Exclusion criteria: 1. Can’t follow the suggestions of a balanced diet 3 days before the experimental process. 2. Consume caffeine, tea, and tobacco and can’t be strictly prohibited during the experimental period. 3. Can’t tolerance the cycling exercise challenge including VO2max test and completed a 60-min cycling exercise at 70% VO2max.

Design outcomes

Primary

Measure	Time frame
1. Glycogen levels in biopsied skeletal muscles are measured using an aseptic technique, an incision (10 mm length and depth) was made in the skin and muscle fascia at about 20 cm above the knee of the right leg at 0-h (immediately after exercise) and 3-h after exercise. 2. Fat oxidation rate in a gaseous sample is measured using MetaMax3B indirect calorimetry (Cortex Biophysik, Nonnenstrasse, Leipzing, Germany) to collect individual’s gaseous samples at every 60-min during 3h post-exercise recovery i.e., at 60, 120 and 180-min after exercise for both trials.	—

Measure

Time frame

1. Glycogen levels in biopsied skeletal muscles are measured using an aseptic technique, an incision (10 mm length and depth) was made in the skin and muscle fascia at about 20 cm above the knee of the right leg at 0-h (immediately after exercise) and 3-h after exercise. 2. Fat oxidation rate in a gaseous sample is measured using MetaMax3B indirect calorimetry (Cortex Biophysik, Nonnenstrasse, Leipzing, Germany) to collect individual’s gaseous samples at every 60-min during 3h post-exercise recovery i.e., at 60, 120 and 180-min after exercise for both trials.

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Secondary

Measure	Time frame
1. p-Akt/Akt ratio is measured using Western blot in muscle biopsied samples at 0-h and 3-h after exercise. 2. GLUT4 expression using Western blot in muscle samples at 0-h and 3-h after exercise. 3. Blood glucose is measured using an automated glucose analyzer supplied by YSI Life Sciences (Yellow Springs, OH, USA) at every 30-min during 3h post-exercise recovery. 4. Insulin is measured using commercially available kits (Randox, Antrim, UK) on an automated analyzer (Hitachi 7020, Tokyo, Japan). at every 30-min during 3h post-exercise recovery. 5. NEFA is measured using commercially available kits (Randox, Antrim, UK) on an automated analyzer (Hitachi 7020, Tokyo, Japan). at every 30-min during 3h post-exercise recovery. 6. Glycerol concentrations are measured using commercially available kits (Randox, Antrim, UK) on an automated analyzer (Hitachi 7020, Tokyo, Japan). at every 30-min during 3h post-exercise recovery.	—

Measure

Time frame

1. p-Akt/Akt ratio is measured using Western blot in muscle biopsied samples at 0-h and 3-h after exercise. 2. GLUT4 expression using Western blot in muscle samples at 0-h and 3-h after exercise. 3. Blood glucose is measured using an automated glucose analyzer supplied by YSI Life Sciences (Yellow Springs, OH, USA) at every 30-min during 3h post-exercise recovery. 4. Insulin is measured using commercially available kits (Randox, Antrim, UK) on an automated analyzer (Hitachi 7020, Tokyo, Japan). at every 30-min during 3h post-exercise recovery. 5. NEFA is measured using commercially available kits (Randox, Antrim, UK) on an automated analyzer (Hitachi 7020, Tokyo, Japan). at every 30-min during 3h post-exercise recovery. 6. Glycerol concentrations are measured using commercially available kits (Randox, Antrim, UK) on an automated analyzer (Hitachi 7020, Tokyo, Japan). at every 30-min during 3h post-exercise recovery.

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Countries

Taiwan

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026