Edentulous subjects wearing full dentures for a minimum period of 1 year. Oral Health
Conditions
Interventions
Volunteers were recruited at the Academic Centre for Dentistry Amsterdam (ACTA) in the Netherlands. Subjects were either patients at the dental clinics or non-professional personnel. All subjects were
Sponsors
Academic Centre for Dentistry Amsterdam (ACTA)
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: edentulous for a minimum period of 1 year wearing a full denture OR dentate subjects with a minimum of 20 teeth.
Exclusion criteria
Exclusion criteria: conditions that are known to systemically affect the neutrophil functionality (such as Diabetes, hematological disorders, recent illness, allergies). Furthermore, antbiotics use, pregnancy, removal partial dentures, night guards, orthodontic banding, oral piercings, oral lesions were excluded.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The presence of functionally impaired neutrophils in the oral cavity of edentulous subjects, measured using flow cytometry directly after sampling. All samples were analyzed using flow cytometry on the same day. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Cell number count, using a Muse cell counting device. 2. Cell membrane integrity, analysed using propidium iodide staining. 3. Neutrophil expression of cell activation markers CD11b, CD63 and CD66b and corrected for non-specific binding of isotype control antibodies. 4. Presence of constitutive reactive oxygen species (ROS) production, detected using dihydrorhodamine 123. 5. Presence of ROS production in response to stimulation, determined in samples incubated with forbid myristate acetate (PMA) or Fusobacterium nucleatum | — |
Countries
Netherlands
Outcome results
None listed