Patients undergoing elective surgery Surgery
Conditions
Interventions
Two blood samples will be taken from elective surgery patients
one before surgery and the second following the completion of the operation. Blood samples will be subjected to laboratory analysis examining the immune and inflammatory response to the surgical inter
Sponsors
University of Birmingham
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Male or female patients aged 18-65 years 2. Patient able to give informed consent 3. Patient undergoing elective open abdominal wall reconstruction surgery
Exclusion criteria
Exclusion criteria: 1. Under the age of 18 years and over the age of 65 years 2. A known malignancy 3. Pregnancy 4. The operation is contaminated (e.g. there is a bowel resection +/- anastomosis, iatrogenic bowel injury or presence of a stoma) 5. Minimally invasive (laparoscopic or robotic) surgery 6. Significant anaesthetic complication (such as anaphylaxis, malignant hyperthermia, negative pressure pulmonary oedema)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The metabolic activity of neutrophils and monocytes isolated from blood samples obtained from elective surgery patients will be measured by flow cytometry, enzyme-linked immunosorbent assays and metabolic tracing before and after their operation | — |
Secondary
| Measure | Time frame |
|---|---|
| The following secondary outcome measures will be assessed in blood samples obtained from elective surgery patients before and after their operations: 1. Anti-microbial activities and surface phenotype of isolated neutrophils, monocytes and/or peripheral blood mononuclear cells (in the absence and/or presence of ex vivo stimulation) will be measured using flow cytometry 2. Circulating markers of inflammation (e.g. pro-inflammatory cytokines, acute phase proteins, cell-free DNA, Damage-associated molecular patterns, whole blood cell counts) will be measured using enzyme-linked immunosorbent assays, whole blood cell haematological analysers and fluorometric-based assays 3. DNA and RNA epigenetic profiles of neutrophils and peripheral blood mononuclear cells RNA sequencing, DNA methylation assays and real-time polymerase chain reaction (RT-PCR) experiments | — |
Countries
England, United Kingdom
Contacts
Public ContactJon Hazeldine
Outcome results
None listed