Semen quality and functionality Other
Conditions
Interventions
Participants will be randomly assigned to one of two intervention groups for 14 weeks:
1. Continue to consume their usual diet supplemented with 60 g/day of a mixture of raw nuts.
2. Continue to consu
Sponsors
INC International Nut and Dried Fruit Council Foundation (Spain)
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: 1. Healthy males 2. 18-35 years old 3. Reported a western-style diet
Exclusion criteria
Exclusion criteria: 1. Frequent consumption of nuts or a known history of allergy 2. Use of plant sterol or fish oil supplements and multivitamins, vitamin E or other antioxidant supplements 3. History of reproductive disorders or vasectomy 4. Current smokers 5. Taking antioxidant supplements or medications for chronic illness 6. Use of illegal drugs
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Improvement of sperm vitality and motility. Semen samples will be collected at baseline and the end of the intervention period. All men will be instructed to abstain for 3 days prior to providing the study specimen, with abstinence period verified by self-report on day of sample collection. Semen samples will be collected allowing analysis within 60 min of sample production. A single researcher, who was blinded as to intervention versus control group status, will perform the conventional semen analyses. For the rest of analysis aliquots of semen will be frozen at -196ºC until analysis. | — |
Secondary
| Measure | Time frame |
|---|---|
| Semen samples will be collected at baseline and the end of the intervention period: 1. Other conventional semen parameters 2. Plasma lipid profile, glucose and insulin concentrations 3. Serum folic acid and alpha-linolenic concentrations 4. Sperm DNA fragmentation 5. Sperm ROS evaluation 6. Sperm miRNA expression profiles 7. Sperm DNA methylation 8. Sperm chromosome stability | — |
Countries
Spain
Outcome results
None listed