Obesity Nutritional, Metabolic, Endocrine Obesity
Conditions
Interventions
Sponsors
Manchester Metropolitan University
Eligibility
Sex/Gender
Male
Age
18 Years to 40 Years
Inclusion criteria
Inclusion criteria: 1. Male. 2. Aged 18-40 years. 3. Non-smokers. 4. Classified as overweight according to body mass index (BMI) class of 25-29.9 kg/m2 and body fat %. 5. Able and willing to comply with study procedures. 6. Willing to undertake required fasting duration, and with the capacity to provide informed consent 7. Free from injury prior to commencing the trials (6+months), with 1+ year free from any injury (such as, lower limb musculoskeletal injuries) that may have prevented and/or affected the participant. from performing cycling previously. 8. Classified as moderate or intermediate chronotypes according to the questionnaire of Waterhouse et al (2001).
Exclusion criteria
Exclusion criteria: 1. Regular medication or any known history of gastrointestinal, respiratory, cardiovascular, or endocrine disease. 2. Early circadian phase also known as extreme morning chronotypes and extreme evening chronotypes. 3. Involved in shift work and report any disturbances to their normal sleep-wake cycle during the two weeks prior to data collection. 4. Cannot consume the test meals due to intolerance's/dietary preferences.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Gastric emptying rate of a semi-solid meal that will be provided 30 minutes following the cessation of the cycling exercise will be measured using the 13C breath test method. 2. Breath samples will be collected every 15 min for a two-hour period. 3. Circulating levels of key gastrointestinal hormones and metabolic markers. Venous blood samples will be collected at baseline, post breakfast ingestion period, 1-hour post breakfast ingestion period (pre-exercise), immediately post 60-min cycle, 30 min post cycle (pre meal ingestion), then at 30 min intervals post meal ingestion for two hours. 4. Subjective feelings of appetite will be assessed using 100 mm visual analogue scales at baseline, post breakfast ingestion period, 30 min post breakfast ingestion period, pre-exercise, post exercise, pre semi-solid meal ingestion, then every 15 min post semi-solid meal ingestion for two hours. 5. 24h post trial energy intake using weighed food intake dietary record. 6. Substrate oxidation will be measured using a breath-by-breath gas analyser at baseline, post breakfast ingestion period, 30 min post breakfast ingestion period, pre-exercise, continuously throughout exercise, pre semi-solid meal ingestion, then every 30 min post semi-solid meal ingestion for two hours. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Salivary melatonin concentration at the start of the trials. 2. Ratings of perceived exertion using the Borg scale and heart rate using telemetry will be recorded pre-exercise and every 15 min during the exercise. | — |
Countries
England, United Kingdom
Contacts
Public ContactVictoria McIver
Outcome results
None listed