Prevention of prolonged sitting-induced deterioration in brain and vascular functions with exercise in apparently healthy adolescents. Not Applicable
Conditions
Interventions
Each participant will make four visits to the laboratory over a 4 to 6-week period, with at least two days separating each visit. While flexible, 2 to 3 days between the sessions are aimed to allow fo
Sponsors
University of Jyväskylä
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Adolescents aged 12-14 years 2. Apparently healthy (no known cardiovascular or metabolic diseases, musculoskeletal injury, serious food allergies)
Exclusion criteria
Exclusion criteria: 1. Metal objects in the body 2. Musculoskeletal injury preventing exercise 3. Cardiovascular or metabolic disease 4. Participating in another study at the same time
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Executive inhibition as a measure of cognitive function is assessed by the flanker task at baseline and 1h, 2h, and 4 h after the baseline 2. Brain activity is measured by combined magnetoencephalography (MEG)–EEG with a 306-channel whole-head neuromagnetometer (Elekta Neuromag TRIUX, Elekta ltd., Stockholm, Sweden) during quiet sitting. First, resting state data as a measure of functional connectivity will be acquired for 10 minutes while the participant sits relaxed in a neuromagnetometer with eyes open. Second, the changes in the magnetic fields and electric potentials in the neural networks in response to the flanker task are assessed. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Arterial stiffness measured by oscillometric tonometry (Arteriograph) and arterial tonometry (PulsePen) is measured in visit 1 and during visits 2-3 at baseline and 2h, and 4h after the baseline 2. Microvascular function, i.e., transient increase in microvascular blood flow after five-minute occlusion, is measured by the laser Doppler methodology (Moor Intruments Ltd) from the left forearm in visit 1 and during visits 2-3 at baseline and 2h, and 4h after the baseline 3. Physical activity and sedentary time are monitored for 72-h prior to the experimental visits using a thigh-worn movement and posture sensor (ActivPALmicro, PALTechonologies). Physical activity and sedentary behaviours are also measured using the PANIC Physical Activity Questionnaire, filled in during visit 1 4. Dietary factors are assessed using one-day dietary records. The participants will note down all food and drink consumed during the previous day prior to the experimental visits. The data will be analysed using the FINELI dietary analysis software (https://fineli.fi/fineli/en/ruokapaivakirja?) 5. Maximal oxygen uptake will be measured during a ramp incremental exercise test with a supramaximal verification phase on a cycle ergometer at visit 1 6. Body composition is estimated using bioelectrical impedance device (InBody 770, Biospace) in visit 1 | — |
Countries
Finland
Contacts
Public ContactEero Haapala
Outcome results
None listed