Spindle transfer for the treatment of infertility problems associated to poor egg quality: a pilot trial

Conditions

Infertility problems associated to impaired embryo development attributed to poor oocyte quality that cannot be overcome with conventional assisted reproduction techniques Pregnancy and Childbirth

Katharina Späth, Nuno Costa-Borges, Eros Nikitos, Konstantinos Kostaras, Gloria Calderón et al. (7 authors)

Human Reproduction2023-06-01

10.1093/humrep/dead093.1049

First pilot study of maternal spindle transfer for the treatment of repeated in vitro fertilization failures in couples with idiopathic infertility.

Costa-Borges N, Nikitos E, Späth K, Miguel-Escalada I, Ma H, Rink K, Coudereau C, Darby H, Koski A, Van Dyken C, Mestres E, Papakyriakou E, De Ziegler D, Kontopoulos G, Mantzavinos T, Vasilopoulos I, Grigorakis S, Prokopakis T, Dimitropoulos K, Polyzos P, Vlachos N, Kostaras K, Mitalipov S, Calderón G, Psathas P, Wells D.

2023-02-1241 citations

10.1016/j.fertnstert.2023.02.008

Interventions

Infertile patients will undergo through a standard in-vitro fertilization cycle with standard follicular stimulation and oocyte pick-up procedures. The collected oocytes from the patient will then be

Eligibility

Sex/Gender

Female

Inclusion criteria

Inclusion criteria: 1. Women under 40 years old 2. Diagnosed with infertility problems associated to impaired embryo development attributed to poor oocyte quality 3. At least two previous in-vitro fertilization (IVF) failed attempts with or without embryo transfer

Exclusion criteria

Exclusion criteria: 1. Women over 40 years old 2. Couples diagnosed with severe male factor infertility

Design outcomes

Secondary

Measure	Time frame
1. Fertilization judged on the next day of the egg pickup and morphokinetic events associated with embryo development evaluated daily during the following 5/6 days of embryo culture in a timelapse incubator 2. Aneuploidy rates judged in biopsied cells from the blastocysts obtained by spindle transfer and measured using advanced genetic molecular tests for screening the chromosome numbers a few days after the blastocyst biopsy 3. Miscarriage and delivery rates calculated based on the total number of patients that will be diagnosed to be pregnant following the criteria described in the primary outcome	—

Measure

Time frame

1. Fertilization judged on the next day of the egg pickup and morphokinetic events associated with embryo development evaluated daily during the following 5/6 days of embryo culture in a timelapse incubator 2. Aneuploidy rates judged in biopsied cells from the blastocysts obtained by spindle transfer and measured using advanced genetic molecular tests for screening the chromosome numbers a few days after the blastocyst biopsy 3. Miscarriage and delivery rates calculated based on the total number of patients that will be diagnosed to be pregnant following the criteria described in the primary outcome

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Primary

Measure	Time frame
1. Blastocyst formation rates evaluated 5/6 days after the egg pickup using a timelapse incubator to monitor the entire embryo development of each embryo until reaching this stage without disturbing the culture conditions 2. Clinical pregnancies and implantation rates judged by elevation of hCG levels and ultrasound confirmation of a gestational sac by 2/3 weeks after embryo transfer. Pregnancies will be further confirmed 2/3 weeks later with fetal heartbeat	—

Measure

Time frame

1. Blastocyst formation rates evaluated 5/6 days after the egg pickup using a timelapse incubator to monitor the entire embryo development of each embryo until reaching this stage without disturbing the culture conditions 2. Clinical pregnancies and implantation rates judged by elevation of hCG levels and ultrasound confirmation of a gestational sac by 2/3 weeks after embryo transfer. Pregnancies will be further confirmed 2/3 weeks later with fetal heartbeat

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Countries

Greece

Contacts

Public ContactEros Nikitos

ivflab@iaso.gr+30 (0)2106184030

Outcome results

None listed