Comparing two ways of cleaning root canals to see which better reduces harmful bacteria during dental treatment

Comparative evaluation of multi-step versus single-step root canal irrigation protocols on reduction of bacterial DNA and RNA levels: a randomized controlled clinical trial

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

ISRCTN

Registry ID

ISRCTN11332195

Enrollment

Registered

2025-11-25

Start date

2024-05-08

Completion date

Unknown

Last updated

2025-12-08

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Maxillary central incisors with necrotic pulp (non-responsive to sensitivity testing), asymptomatic apical periodontitis Oral Health

Interventions

Procedure and Bacterial Sampling: 28 patients included, All teeth follow strict aseptic measures, including plaque removal and disinfection with 30% H2O2 followed by 2.5% NaOCl, which is then inactiva

Eligibility

Sex/Gender

All

Age

18 Years to 60 Years

Inclusion criteria

Inclusion criteria: 1. Maxillary central incisors with necrotic pulp (non-responsive to sensitivity testing) 2. Asymptomatic apical periodontitis, confirmed by periapical radiographs 3. Patients aged 18–60 years

Exclusion criteria

Exclusion criteria: 1. Symptomatic teeth (pain, tenderness, swelling) 2. Immature roots 3. Antibiotic use within 3 months 4. Systemic disease 5. Inability to isolate with a rubber dam 6. Periodontal pockets >3 mm 7. Root fractures 8. Severe decay

Countries

Iraq

Contacts

Public ContactOmar Khaleefah

omr_ta@yahoo.com+9647816788826

Outcome results

None listed

Source: ISRCTN (via WHO ICTRP) · Data processed: Feb 4, 2026