Exercise-induced increases in gut permeability Digestive System
Conditions
Interventions
1. Trial whey protein concentrate
2. Placebo (also whey-based protein)
Treatments will be administered using a randomised, double-blind design. Participants will consume 40 g of their assigned protei
Sponsors
University of Kent
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: 1. Male or female 2. 18-65 years old
Exclusion criteria
Exclusion criteria: 1. Disease of the GI tract 2. Renal, hepatic, endocrine (including diabetes mellitus), cardiac, pulmonary, pancreatic, neurologic or biliary disorders 3. Intolerance or any known or suspected hypersensitivity (allergy) to study products/ingredients 4. Recent use of antibiotics (within 1 month of study) 5. Current, or recent, use of certain medications (NSAIDs) 6. Current, or recent, use of certain supplements (as per the researcher's determination) that could influence study measures or outcomes (such as pre or probiotics, for example) 6. Females who are pregnant, lactating, or planning to become pregnant during the study period 7. Enrolment in any other similar trial within 3 months prior to recruitment 8. Investigator's determination of unsuitability for trial participation
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Lactulose:rhamnose ratio will be measured in urine samples taken at baseline (pre-supplementation) and post-exercise on main trials | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Intestinal fatty-acid binding protein (I-FABP) will be measured by ELISA assays in blood samples taken pre-, post- and +1 h post-exercise 2. Bacterial load will be measured by qPCR on blood samples taken pre-, post- and +1 h post-exercise 3. Soluble CD14 (sCD14) concentration will be measured by ELISA assays in blood samples taken pre-, post- and +1 h post-exercise 4. WBC differentiation will be immediately performed on whole blood samples taken pre-, post- and 1-h post-exercise on main trial days | — |
Countries
England, United Kingdom
Contacts
Public ContactWilliam Searle
Outcome results
None listed