Effect of a polyphenol-rich diet on leaky gut in the elderly

Conditions

Intestinal permeability (IP) Digestive System Intestinal permeability (IP)

Marino M, Del Bo' C, Martini D, Perna S, Porrini M, Cherubini A, Gargari G, Meroño T, Hidalgo-Liberona N, Andres-Lacueva C, Kroon PA, Guglielmetti S, Riso P.

2024-08-244 citations

10.1186/s12877-024-05272-y

A Polyphenol‐Rich Diet Increases the Gut Microbiota Metabolite Indole 3‐Propionic Acid in Older Adults with Preserved Kidney Function

Gregorio Peron, Tomás Meroño, Giorgio Gargari, Nicole Hidalgo‐Liberona, Antonio Miñarro et al. (15 authors)

Molecular Nutrition & Food Research2022-03-2236 citations

10.1002/mnfr.202100349

Higher bacterial DNAemia can affect the impact of a polyphenol-rich dietary pattern on biomarkers of intestinal permeability and cardiovascular risk in older subjects.

Gargari G, Taverniti V, Del Bo' C, Bernardi S, Hidalgo-Liberona N, Meroño T, Andres-Lacueva C, Kroon PA, Cherubini A, Riso P, Guglielmetti S.

2021-11-0211 citations

10.1007/s00394-021-02680-3

Crosstalk among intestinal barrier, gut microbiota and serum metabolome after a polyphenol-rich diet in older subjects with “leaky gut”: The MaPLE trial

Gregorio Peron, Giorgio Gargari, Tomás Meroño, Antonio Miñarro, Esteban Vegas et al. (16 authors)

Clinical Nutrition2021-09-0961 citations

10.1016/j.clnu.2021.08.027

A polyphenol-rich dietary pattern improves intestinal permeability, evaluated as serum zonulin levels, in older subjects: The MaPLE randomised controlled trial.

Del Bo' C, Bernardi S, Cherubini A, Porrini M, Gargari G, Hidalgo-Liberona N, González-Domínguez R, Zamora-Ros R, Peron G, Marino M, Gigliotti L, Winterbone MS, Kirkup B, Kroon PA, Andres-Lacueva C, Guglielmetti S, Riso P.

2020-12-1887 citations

10.1016/j.clnu.2020.12.014

Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial.

Guglielmetti S, Bernardi S, Del Bo' C, Cherubini A, Porrini M, Gargari G, Hidalgo-Liberona N, Gonzalez-Dominguez R, Peron G, Zamora-Ros R, Winterbone MS, Kirkup B, Kroon PA, Andres-Lacueva C, Riso P.

2020-02-2641 citations

10.1186/s12877-020-1472-9

Interventions

Based on a computer randomization plan, subjects are randomized to receive the two study diets in a random order. Participants follow the diets for eight weeks, followed by an eight week washout perio

Eligibility

Sex/Gender

All

Inclusion criteria

Inclusion criteria: 1. Age > 60 years old 2. Intestinal Permeability evaluated by Zonulin serum level 3. Adequate nutritional status evaluated with Mini Nutritional Assessment (MNA) score =24 4. Good cognitive status tested with Mini Mental State Evaluation (MMSE) score =24 5. Self-sufficiency assessed with validated tests (e.g. Barthel index - activities of daily living, Tinetti balance assessment)

Exclusion criteria

Exclusion criteria: 1. Celiac disease 2. Severe liver disease with cirrhosis 3. Severe renal insufficiency (dialysis) 4. Presence of Chronic Obstructive Pulmonary severe COPD (oxygen therapy for many hours a day) or severe heart failure, i.e. class III or IV NYHA - New York Heart Association 5. Antibiotic treatment 6. Malignant tumor that required treatment in the previous 2 years

Design outcomes

Primary

Measure	Time frame
Intestinal permeability is evaluated by measuring Zonulin serum levels using an ELISA kit at baseline, 8, 16 and 24 weeks.	—

Secondary

Measure	Time frame
1. Fecal microbiota composition evaluated by 16S rRNA gene profiling with MiSeq-Illumina platform at baseline, 8, 16 and 24 weeks 2. Short chain fatty acids and polyphenol-derived metabolites measured with LC-MS system at baseline, 8, 16 and 24 weeks 3. Diet-induced changes in microbiota metabolism performed by NMR at baseline, 8, 16 and 24 weeks 4. LC-MS analysis are used for faecal water at baseline, 8, 16 and 24 weeks 5. Total blood bacterial load are assessed through a quantitative real-time PCR; taxomic profile investigated by 16S rRNA gene profiling with MiSeq-Illumina platform at baseline, 8, 16 and 24 weeks 6. Inflammatory markers, oxidative stress and related markers e.g. vascular cell adhesion molecule (VCAM)-1 and intracellular adhesion molecule (ICAM)-1 performed through ELISA kit at baseline, 8, 16 and 24 weeks 7. DNA damage assessed through Comet Assay at baseline, 8, 16 and 24 weeks 8. Endotoxin by Limulus Amebocyte Lysate (LAL) assay assessed to evaluate the inflammatory process and oxidative stress modulation at baseline, 8, 16 and 24 weeks 9. Metabolomic analysis will be performed on HPLC-ESI-Q-ToF-MS and NMR to understand the interaction of the polyphenol rich diet with the secondary metabolites of microbiota at baseline, 8, 16 and 24 weeks	—

Measure

Time frame

1. Fecal microbiota composition evaluated by 16S rRNA gene profiling with MiSeq-Illumina platform at baseline, 8, 16 and 24 weeks 2. Short chain fatty acids and polyphenol-derived metabolites measured with LC-MS system at baseline, 8, 16 and 24 weeks 3. Diet-induced changes in microbiota metabolism performed by NMR at baseline, 8, 16 and 24 weeks 4. LC-MS analysis are used for faecal water at baseline, 8, 16 and 24 weeks 5. Total blood bacterial load are assessed through a quantitative real-time PCR; taxomic profile investigated by 16S rRNA gene profiling with MiSeq-Illumina platform at baseline, 8, 16 and 24 weeks 6. Inflammatory markers, oxidative stress and related markers e.g. vascular cell adhesion molecule (VCAM)-1 and intracellular adhesion molecule (ICAM)-1 performed through ELISA kit at baseline, 8, 16 and 24 weeks 7. DNA damage assessed through Comet Assay at baseline, 8, 16 and 24 weeks 8. Endotoxin by Limulus Amebocyte Lysate (LAL) assay assessed to evaluate the inflammatory process and oxidative stress modulation at baseline, 8, 16 and 24 weeks 9. Metabolomic analysis will be performed on HPLC-ESI-Q-ToF-MS and NMR to understand the interaction of the polyphenol rich diet with the secondary metabolites of microbiota at baseline, 8, 16 and 24 weeks

—

Countries

Italy

Outcome results

None listed