Varicocele.
Conditions
Interventions
Intervention 1: Recipient of the probiotic Lactobacillus rhamnosus. Intervention 2: Intervention group: Vitamin E recipient. Intervention 3: Control group: Placebo (dextrose) recipient.
Sponsors
Shiraz University of Medical Sciences
Eligibility
Sex/Gender
Male
Age
18 Years to 45 Years
Inclusion criteria
Inclusion criteria: People who refer to the Hazrat Ali Asghar Shiraz Medical Center will have their disease diagnosed by a single surgeon with Doppler ultrasound and will undergo surgery Patients must be between the ages of 18 and 45 The patient's BMI must be between 18 and 25 Clinical diagnosis must be grade two or three varicocele on the left side Patients must be candidate for subinguinal varicocelectomy
Exclusion criteria
Exclusion criteria: consumption of any kinds of antibiotics or probiotics in the past month patients with infectious diseases and AIDS Having metabolic and digestive diseases that affect fertility Having hormonal disorders History of testicular cancer History of smoking, alcohol, drugs, and psychotropic substances Special diets Allergy to probiotics Patients with azoospermia Patients with testicular atrophy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sperm parameters (variables of semen volume, concentration, motility and sperm kinematics); sperm viability; sperm DNA fragmentation index (DFI); normal sperm morphology; sperm acrosomal reaction; oxidative stress indices (TAC, MDA, GPx, SOD and catalase); serum levels of total and free testosterone. Timepoint: Measurement of semen parameters before the start of the study and three months after taking the drug; measurement and comparison of sperm viability, sperm DNA fragmentation (DFI), with normal sperm morphology, sperm acrosome reaction, oxidative stress indicators (TAC, MDA, GPx, SOD and catalase) and serum levels of total and free testosterone after three months of taking the drug. Method of measurement: Semen analysis with a light microscope and CASA computerized sperm analyzer; sperm viability assessment with eosin staining and light microscopy; DNA structure integrity assessment using a sperm chromatin dispersion kit; sperm morphology assessment with a Diff-Quik kit; acrosome reaction assessment using FITC-PSA lectin and fluorescent microscopy with FITC filter; measurement of oxidative stress indices (TAC, MDA, GPx, SOD, and catalase) and serum levels of total and free testosterone with commercial ELISA kits. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMohammad Reza Zarei
Shiraz University of Medical Sciences
Outcome results
None listed