healthy individuals.
Conditions
Interventions
Intervention 1: Intervention group: Participants in this group perform a 12-week progressive resistance training program, 3 sessions per week, 40 minutes per session. The exercises include: barbell be
Sponsors
Islamic Azad University
Eligibility
Sex/Gender
Male
Age
25 Years to 45 Years
Inclusion criteria
Inclusion criteria: Having a history of regular resistance training for at least the past six month No consumption of any sports supplements or anti-inflammatory drugs in the three months prior to the study No history of musculoskeletal diseases or injuries (e.g., metabolic, joint, or orthopedic problems). Age range: 25 to 45 years Sex: Male Body Mass Index (BMI): 18.5 to 24.9 (normal range)
Exclusion criteria
Exclusion criteria: Medical conditions: Metabolic diseases (e.g., diabetes), cardiovascular, renal, hepatic, or chronic inflammatory diseases Musculoskeletal problems Any acute/chronic injury, recent limb surgery, or orthopedic issues preventing proper resistance training execution Medication/Supplement use: Use of anti-inflammatory drugs, corticosteroids, or sports supplements (protein, creatine, etc.) within 3 months prior to the study Substance use: Smoking (cigarettes/hookah) within the past 6 months, or regular alcohol consumption Concurrent participation: Enrolled in other research studies simultaneously Other: Unwillingness to comply with the study protocol, or inability to provide written informed consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| MMP2 (rs3918242) gene expression. Timepoint: Before the intervention (baseline). 48 hours after the last training session (end of week 12). Method of measurement: RNA extraction from peripheral blood lymphocytes, cDNA synthesis, then measurement by Real-time PCR using SYBR Green method. Data are normalized to GAPDH reference gene using the ??Ct method.;MMP9 (rs2285053) gene expression. Timepoint: Before the intervention (baseline). 48 hours after the last training session (end of week 12). Method of measurement: RNA extraction from peripheral blood lymphocytes, cDNA synthesis, then measurement by Real-time PCR using SYBR Green method. Data are normalized to GAPDH reference gene using the ??Ct method.;COL1A1 gene expression. Timepoint: Before the intervention (baseline). 48 hours after the last training session (end of week 12). Method of measurement: RNA extraction from peripheral blood lymphocytes, cDNA synthesis, then measurement by Real-time PCR using SYBR Green method. Data are normalized to GAPDH reference gene using the ??Ct method.;Serum creatine kinase (CK) level. Timepoint: Before the intervention (baseline). 48 hours after the last training session (end of week 12). Method of measurement: Blood sampling from antecubital vein, serum separation, then measurement by ELISA (Human CK ELISA Kit) with optical density reading at 450 nm wavelength.;Serum interleukin-6 (IL-6) level. Timepoint: Before the intervention (baseline). 48 hours after the last training session (end of week 12). Method of measurement: Blood sampling from antecubital vein, serum separation, then measurement by ELISA (Human IL-6 Quantikine ELISA Kit) with optical density reading at 450 nm wavelength.;Serum C-reactive protein (CRP) level. Timepoint: Before the intervention (baseline). 48 hours after the last training session (end of week 12). Method of measurement: Blood sampling from antecubital vein, serum separation, then measurement by ELISA (Human CRP ELISA Kit) with optical density readin | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactShohreh Naemi
Islamic Azad University
Outcome results
None listed