Effect of Probiotics in Obesity

The effect of eight weeks of high-intensity interval training and probiotic supplementation on the abundance of selected gut microbiota in obese women

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20251211068287N2

Enrollment

Registered

2026-02-08

Start date

2026-02-17

Completion date

Unknown

Last updated

2026-06-01

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Obesity.

Interventions

Intervention 1: Intervention Group 1 (Probiotic): Daily intake of 1 probiotic capsule for 8 weeks

Intervention Group 2 (Exercise): High-Intensity Interval Training (HIIT) program on a stationary cycle, 3 sessions per week for 8 weeks

Intervention Group 3 (Combination): Concurrent receipt of both Intervention 1 and Intervention 2 in full. Intervention 2: Control group: No intervention (usual lifestyle)

Eligibility

Sex/Gender

Female

Age

30 Years to 40 Years

Inclusion criteria

Inclusion criteria: Age range 30–40 years body mass index (BMI) greater than 29.9 and less than 34.99 kg/m²

Exclusion criteria

Exclusion criteria: Diagnosis of inflammatory bowel disease (Crohn's disease, ulcerative colitis), active celiac disease, or severe irritable bowel syndrome (IBS). Regular use of probiotics, prebiotics, or fiber supplements within the past 1 month.

Design outcomes

Primary

Measure	Time frame
Gut Microbiome Abundance. Timepoint: Before and after the exercise protocol. Method of measurement: Gut microbiome abundance will be measured by collecting stool samples before and after the intervention. Samples will be stored under standard conditions, DNA will be extracted, and 16S rRNA sequencing will be performed to determine the composition and relative abundance of bacterial taxa. For key species, quantitative PCR (qPCR) will be used to estimate absolute abundance. Results will be reported as changes between pre- and post-intervention and across study groups.	—

Measure

Time frame

Gut Microbiome Abundance. Timepoint: Before and after the exercise protocol. Method of measurement: Gut microbiome abundance will be measured by collecting stool samples before and after the intervention. Samples will be stored under standard conditions, DNA will be extracted, and 16S rRNA sequencing will be performed to determine the composition and relative abundance of bacterial taxa. For key species, quantitative PCR (qPCR) will be used to estimate absolute abundance. Results will be reported as changes between pre- and post-intervention and across study groups.

—

Secondary

Measure	Time frame
Inflammation index. Timepoint: Before and after the exercise protocol. Method of measurement: Inflammatory markers will be measured using fasting venous blood samples. After serum or plasma separation and storage at -80 °C, levels of pro- and anti-inflammatory cytokines (such as TNF-a, IL-6, and IL-10) and C-reactive protein will be determined by standard ELISA or multiplex assays. Results will be reported as changes between pre- and post-intervention and across study groups.	—

Measure

Time frame

Inflammation index. Timepoint: Before and after the exercise protocol. Method of measurement: Inflammatory markers will be measured using fasting venous blood samples. After serum or plasma separation and storage at -80 °C, levels of pro- and anti-inflammatory cytokines (such as TNF-a, IL-6, and IL-10) and C-reactive protein will be determined by standard ELISA or multiplex assays. Results will be reported as changes between pre- and post-intervention and across study groups.

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactRostam Alizadeh

ILAM University

r.alizadeh@ilam.ac.ir+98 84 5924 1000

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Jun 11, 2026