Dental caries is a chronic, biofilm-mediated disease characterized by demineralization of tooth hard tissues caused by acidogenic bacteria. It remains one of the most prevalent oral diseases globally. This study investigates preventive strategies against caries through the short-term release of chitosan from a topical dental varnish in healthy adult volunteers. Although participants were not patients, the research focuses on caries prevention and salivary protective factors related to caries ris
Conditions
Interventions
Intervention group: Name of group: Chitosan varnish 5% groupDefinition and formulation:Each participant received a varnish prepared from synthetic chitosan powder (3 g) dissolved in 9 mL of acetic aci
sampling for saliva was done 30 min, 60 min, and 1 month after the single application.Manufacturer:The varnish was laboratory-prepared at the Faculty of Dentistry, Hamadan University of Medical Scienc
Sponsors
Hamedan University of Medical Sciences
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Good oral hygiene, at least 24 sound teeth No use of antibiotics in the last 6 months Non-smokers Not using mouthwash in the last week No orthodontic or fixed dental prosthesis No systemic disease Provided written informed consent
Exclusion criteria
Exclusion criteria: Periodontal disease Systemic disease Pregnancy Cleft lip/palate or craniofacial anomalies Refusal or withdrawal of consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in salivary pH following the application of 5% chitosan varnish, compared with baseline values, to evaluate the short-term effect of the varnish on oral environment acidity. Timepoint: Measured at basedline (before varnish), 30 minutes, 60 minutes, and one month after. Method of measurement: Salivary pH was measured using the strip method (pH test strips), comparing color changes with the manufacturer’s reference chart. | — |
Secondary
| Measure | Time frame |
|---|---|
| Concentration of chitosan released into saliva after application of 5% chitosan varnish, to evaluate the varnish’s release profile and its potential for sustained delivery in the oral cavity. Timepoint: Before intervention, 30 minutes, 60 minutes, 1 month after. Method of measurement: Salivary chitosan concentration was determined using the ninhydrin colorimetric method, with absorbance measured at 570 nm by spectrophotometer. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactZahra Khamverdi
Hamedan University of Medical Sciences
Outcome results
None listed