Muscular atrophy.
Conditions
Interventions
Intervention 1: Intervention group: In this study, the non-dominant hand (left hand) of all study subjects will be immobilized with a fiberglass half splint for 8 days. The intervention group will par
Sponsors
Shiraz University of Medical Sciences
Eligibility
Sex/Gender
All
Age
18 Years to 40 Years
Inclusion criteria
Inclusion criteria: Non-athletes Healthy Right handed Normal BMI Normal body fat percentage No history of resistance exercise in the 6 months prior to the start of the study
Exclusion criteria
Exclusion criteria: History of cardiovascular diseases History of limb fractures History of metabolic diseases History of neuromuscular diseases History of MS History of muscular dystrophy Movement problems
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Cross-sectional area of slow and fast twitch muscle fibers of the biceps brachii muscle. Timepoint: 2 days before the start of the intervention and 1 day after the end of the intervention. Method of measurement: To measure the cross-sectional area of ??muscle fibers, tissue histology (staining) is used, and the structure of muscle cells and their changes are examined under a microscope. First, 25 mg of muscle will be taken from the center of the long head of the biceps brachii muscle using an 18-gauge Japanese fully automatic biopsy needle. After sampling, the harvested tissue will be placed in a 10% formalin solution and transported to the laboratory for tissue staining tests. In the laboratory, cross-sections of muscle tissues will first be stained with hematoxylin, then the structure of muscle cells and their changes will be clearly studied under a microscope.;Muscle RING-finger protein-1 (MuRF1) gene expression in the biceps brachii muscle. Timepoint: 2 days before the start of the intervention and 1 day after the end of the intervention. Method of measurement: To measure the expression of the Muscle RING-finger protein-1 gene, Real-Time PCR or real-time polymerase chain reaction will be used. Initially, 25 mg of muscle will be taken from the center of the long head of the biceps brachii muscle through a fully automated 18-gauge Japanese biopsy needle. After sampling, the harvested tissue is frozen in a nitrogen tank and transferred to the laboratory for Real-Time PCR tests. This laboratory method consists of several steps. Initially, DNA or RNA samples are extracted and prepared from biological samples. Then, a mixture is prepared for DNA/RNA amplification. The resulting mixture is transferred to the Real-time PCR device and the DNA/RNA amplification process begins. At this stage, the Real-time PCR device simultaneously measures the fluorescence of the amplified DNA/RNA. The fluorescence data collected by the Real-time PCR device are analyzed to calculate the a | — |
Secondary
| Measure | Time frame |
|---|---|
| Maximum Voluntary Isometric Contraction (MVIC). Timepoint: 2 days before the start of the intervention and 1 day after the end of the intervention. Method of measurement: To measure the size of isometric voluntary contraction, the skin electromyography method is used. First, an electrode is placed on the skin and the subject is asked to contract their biceps muscle with maximum force. The electrodes then measure the nerve impulses entering the muscle through the motor neurons.;One repetition maximum strength (1RM strength). Timepoint: 2 days before the start of the intervention and 1 day after the end of the intervention. Method of measurement: To measure the strength of one repetition maximum, a field test and a 1RM calculation formula are used. First, we select a weight for the individual that he can move between 10-20 repetitions. Then, according to the amount of weight and the number of repetitions, the strength of one repetition maximum is measured through the formula. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactAlireza Ostovar
University of Guilan
Outcome results
None listed