Investigation of serum ferritin and transferrin levels in the iron absorption capacity ofthalassemia minor patients after a period of endurance training and consumption ofsoy protein isolate

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20250420065403N1

Enrollment

Registered

2026-01-21

Start date

2025-04-19

Completion date

Unknown

Last updated

2026-02-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Factors affecting the hematological status of thalassemia minor.

Interventions

Control group:.

Eligibility

Sex/Gender

All

Age

25 Years to 40 Years

Inclusion criteria

Inclusion criteria: Definitive diagnosis of thalassemia based on hematological tests Age between 25 and 40 years Lack of participation in regular sports activities in the past 6 months Not taking iron supplements and related medications in the past 3 months No smoking or alcohol consumption Not pregnant or breastfeeding Willingness to cooperate and signing a consent form

Exclusion criteria

Exclusion criteria: Unwillingness to continue cooperation at any time during the research The occurrence of any side effects Use of medications that affect iron absorption or metabolism The emergence of any new diseases that may affect the outcome of the study

Design outcomes

Primary

Measure	Time frame
The effect of endurance training and soy protein isolate supplementation on transferrin levels in patients with thalassemia minor "Percentage of people with thalassemia minor". Timepoint: Before starting training and taking supplements, week 3 and week 6. Method of measurement: Serum transferrin Serum transferrin was measured by immunoturbidimetric method using Cobas C311 and Roche kit. The required serum sample volume was 0.5 ml, which was separated from the previous sample. According to the manufacturer's protocol, each series of measurements was started using calibration standards and positive and negative controls. Each sample was read in duplicate and if the difference was more than 5%, the sample was re-examined.;The effect of endurance training and soy protein isolate supplementation on ferritin levels in patients with thalassemia minor " Percentage of people with thalassemia minor ". Timepoint: Before starting training and taking supplements, week 3 and week 6. Method of measurement: To measure serum ferritin, at each of the three time points before the intervention, at the end of the third week, and at the end of the sixth week, 5 ml of venous blood was collected from the brachial vein after 12 hours of fasting with a 22G syringe and needle by a laboratory expert. The sample was immediately transferred to the central laboratory and centrifuged at 3000 rpm for 10 minutes. The separated serum was transferred to sterile microtubes and stored in a -20°C freezer until measurement. Ferritin was measured by ELISA using a Roche kit and a Stat Fax 3200 device, based on IFCC standards. Calibration of the device before each series of tests, use of internal and external controls ( patients with borderline and normal ferritin levels ), repetition of the measurement of each suspicious sample, and recording all steps in the quality control office were among the requirements of the measurement.;The effect of endurance training and soy protein isolate supplementation on hem	—

Measure

Time frame

The effect of endurance training and soy protein isolate supplementation on transferrin levels in patients with thalassemia minor "Percentage of people with thalassemia minor". Timepoint: Before starting training and taking supplements, week 3 and week 6. Method of measurement: Serum transferrin Serum transferrin was measured by immunoturbidimetric method using Cobas C311 and Roche kit. The required serum sample volume was 0.5 ml, which was separated from the previous sample. According to the manufacturer's protocol, each series of measurements was started using calibration standards and positive and negative controls. Each sample was read in duplicate and if the difference was more than 5%, the sample was re-examined.;The effect of endurance training and soy protein isolate supplementation on ferritin levels in patients with thalassemia minor " Percentage of people with thalassemia minor ". Timepoint: Before starting training and taking supplements, week 3 and week 6. Method of measurement: To measure serum ferritin, at each of the three time points before the intervention, at the end of the third week, and at the end of the sixth week, 5 ml of venous blood was collected from the brachial vein after 12 hours of fasting with a 22G syringe and needle by a laboratory expert. The sample was immediately transferred to the central laboratory and centrifuged at 3000 rpm for 10 minutes. The separated serum was transferred to sterile microtubes and stored in a -20°C freezer until measurement. Ferritin was measured by ELISA using a Roche kit and a Stat Fax 3200 device, based on IFCC standards. Calibration of the device before each series of tests, use of internal and external controls ( patients with borderline and normal ferritin levels ), repetition of the measurement of each suspicious sample, and recording all steps in the quality control office were among the requirements of the measurement.;The effect of endurance training and soy protein isolate supplementation on hem

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactRoya zohrabi

Islamic Azad University

roya.zohrabi7987@gmail.com+98 933 343 8982

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 7, 2026