Immunosenescence in CD8 and CD4 cell subsets.
Conditions
Interventions
Intervention 1: Intervention group: Recreational football training group. Participants in this group will engage in small-sided recreational football training for 12 weeks, two sessions per week. Each
Sponsors
Football & NCDs Research Core, University of Isfahan
Eligibility
Sex/Gender
Male
Age
50 Years to 70 Years
Inclusion criteria
Inclusion criteria: Good health Lack of regular exercise other than daily activities for at least the past 2 years Stable body weight (±5 kg) for a minimum of 6 months prior to enrollment. Age 50 or older
Exclusion criteria
Exclusion criteria: Cardiovascular disease, cancer, diabetes, thyroid disease, osteoporosis, uncontrolled hypertension, cognitive impairment, lung disease, kidney disease, and immunological diseases (e.g., autoimmune diseases). Immunosuppressant medication use Smoking, alcohol, drug, or medication addiction Concurrent participation in another clinical study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Frequency of terminally differentiated effector memory CD8+ T cells (TEMRA). Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Flow cytometric analysis of peripheral blood samples.;Frequency of effector memory CD8+ T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Flow cytometric analysis of peripheral blood samples.;Frequency of central memory CD8+ T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Flow cytometric analysis of peripheral blood samples.;Frequency of naïve CD8+ T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Flow cytometric analysis of peripheral blood samples.;The ratio of peripheral CD4? T-cells to CD8? T-cells (CD4?/CD8?). Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Flow cytometric analysis of peripheral blood samples. | — |
Secondary
| Measure | Time frame |
|---|---|
| Correlation between changes in VO2max and the frequency of peripheral CD8+ effector memory T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Pearson correlation coefficient or Spearman's rank correlation coefficient.;Correlation between changes in VO2max and the frequency of peripheral CD8+ central memory T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Pearson correlation coefficient or Spearman's rank correlation coefficient.;Correlation between changes in VO2max and the frequency of peripheral CD8+ naive T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Pearson correlation coefficient or Spearman's rank correlation coefficient.;Correlation between changes in VO2max and the CD4+/CD8+ ratio. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Pearson correlation coefficient or Spearman's rank correlation coefficient.;Correlation between changes in VO2max and the frequency of peripheral CD8+ TEMRA T cells. Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Pearson correlation coefficient or Spearman's rank correlation coefficient.;Maximal oxygen uptake (VO2max). Timepoint: Baseline (24 hours before the intervention) and post-intervention (24 hours after the final session of the 12-week training program). Method of measurement: Rockport 1 mile walk test. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactReza Moqimi
Faculty of Sport Sciences University of Isfahan
Outcome results
None listed