The effect of eight weeks of HIIT trainings and probiotic supplementation on gene expression of receptors and plasma concentration of serotonin, and heart rate variability in sedentary young women. Seeking and accepting physical, nutritional and chemical interventions known to be hazardous and harmful
Conditions
Interventions
Intervention 1: First intervention group: The first group will perform high-intensity interval training for eight weeks, three days (sessions) per week, for approximately 45 minutes per session, along
Sponsors
The University of Tabriz
Eligibility
Sex/Gender
Female
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: female gender age range of 20-40 years Having no exercise training for at least six months
Exclusion criteria
Exclusion criteria: Having a history of neurological diseases or autonomic nervous system disorders Cardiovascular diseases Respiratory diseases Metabolic diseases Having orthopedic problems
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Serum serotonin concentration. Timepoint: One day before and one day after eight weeks of interventions that included exercise and probiotic supplementation. Method of measurement: A German-made LDN ELISA kit and an ELISA reader will be used to measure the quantitative concentration of serum serotonin.;Blood serotonin receptor gene expression (5-HTR2A). Timepoint: One day before and one day after eight weeks of interventions that included exercise and probiotic supplementation. Method of measurement: Real-time polymerase chain reaction will be used to measure gene expression.;Serotonin transporter gene expression (SERT). Timepoint: One day before and one day after eight weeks of interventions that included exercise and probiotic supplementation. Method of measurement: Real-time polymerase chain reaction will be used to measure gene expression.;Heart rate variability. Timepoint: One day before and one day after eight weeks of interventions that included exercise and probiotic supplementation. Method of measurement: A three-channel Holter monitor system, from Cantec company, model 9803TLC, made in China, will be used to measure heart rate variability. | — |
Secondary
| Measure | Time frame |
|---|---|
| Serum cortisol concentration. Timepoint: One day before and one day after eight weeks of exercise interventions and probiotic supplementation. Method of measurement: ELISA method by Monobind kit made in the USA.;Profile of mood state. Timepoint: One day before and one day after eight weeks of exercise interventions and probiotic supplementation. Method of measurement: Profile Of Mood State Questionnaire (Modified), 40 questions.;Body composition (fat mass, muscle mass). Timepoint: One day before and one day after eight weeks of exercise interventions and probiotic supplementation. Method of measurement: Body Composition Scale, Xiaomi, S400-MJTZC01YM.;Blood Pressure. Timepoint: One day before and one day after eight weeks of exercise interventions and probiotic supplementation. Method of measurement: Omron digital blood pressure monitor. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactZakeri, Noushin
The University of Tabriz
Outcome results
None listed