Comparison of expression levels of BMP15, GDF9, FLT1 and HIF1 genes in granulosa cells of women with poor ovarian response

Comparing the expression levels of BMP15, GDF9, FLT1 and HIF1 genes in granulosa cells of women with poor ovarian response under GCSF treatment with women with poor ovarian response receiving normal saline: a clinical trial

Status

Recruiting

Phases

Phase 2

Study type

Interventional

Source

IRCT

Registry ID

IRCT20241218064088N1

Enrollment

Registered

2025-02-03

Start date

2025-01-29

Completion date

Unknown

Last updated

2025-07-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Poor ovarian response. Female infertility associated with anovulation

Interventions

Intervention 1: Intervention group: G-CSF (Granulocyte-colony stimulating factor) - 0.25 ml Filgrastim 300 mcg/0.5ml. Subcutaneous once every other day from day 1 to day 3 of the menstrual cycle until

Eligibility

Sex/Gender

Female

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: Age group 20-40 years POSEIDON poor responder group 3 and 4 (expected poor responder group with AFC <5 and antimüllerian hormone (AMH) less than 1.1 ng/ml) Ovulation induction by GnRH antagonist protocol Normal parameters of semen Sperm intracytoplasmic injection Willingness to participate in the study and provide written informed consent Injection of platelet-rich plasma into the ovary

Exclusion criteria

Exclusion criteria: Contraindications for intracytoplasmic sperm injection(ICSI) treatment Contraindications to the use of granulocyte colony-stimulating factor and dydrogesterone Inability to fully comply with the study protocol In the last 3 months, G-CSF has been used for treatment

Design outcomes

Primary

Measure	Time frame
GDF9 gene expression level in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of GDF9 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;BMP15 gene expression level in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of BMP15 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;The level of FLT1 gene expression in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of FLT1 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;HIF1 gene expression level in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of HIF1 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;Number of mature oocyte. Timepoint: After collecting the oocytes. Method of measurement: Counting in the laboratory.;Follicular fluid AMH. Timepoint: At the end of the study and after collecting the follicular fluid of the total sample volume. Method of measurement: Using the kit.;Follicular fluid FSH. Timepoint: At the end of the study and after collecting the follicular fluid of the total sample volume. Method of measurement: Using the kit.	—

Measure

Time frame

GDF9 gene expression level in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of GDF9 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;BMP15 gene expression level in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of BMP15 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;The level of FLT1 gene expression in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of FLT1 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;HIF1 gene expression level in granulosa cells in GCSF and normal saline treated groups. Timepoint: The measurement of HIF1 gene expression in granulosa cells is at the end of the study and after collecting the follicular fluid of the entire sample volume. Method of measurement: Polymerase Chain Reaction.;Number of mature oocyte. Timepoint: After collecting the oocytes. Method of measurement: Counting in the laboratory.;Follicular fluid AMH. Timepoint: At the end of the study and after collecting the follicular fluid of the total sample volume. Method of measurement: Using the kit.;Follicular fluid FSH. Timepoint: At the end of the study and after collecting the follicular fluid of the total sample volume. Method of measurement: Using the kit.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactMehrnoosh Amanatbehbahani

Shiraz University of Medical Sciences

amanatbehbahani@sums.ac.ir+98 71 3231 5768

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026