Blood Flow Restriction Resistance Training, Follistatin, and IGF-1

The Effects of Resistance Training With and Without Blood Flow Restriction on Follistatin and IGF-1 Levels in Male Students

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20241205063953N2

Enrollment

Registered

2025-02-09

Start date

2025-01-29

Completion date

Unknown

Last updated

2025-07-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Exercise. Encounter for examination for adolescent development state

Interventions

Intervention 1: Intervention group: Participants will train for eight weeks, three sessions per week. Training intensity will be set at 20-30% of one-repetition maximum (1RM), with specialized cuffs a

Eligibility

Sex/Gender

Male

Age

20 Years to 25 Years

Inclusion criteria

Inclusion criteria: Male aged 20 to 25 years Body Mass Index (BMI) between 20 and 25 kg/m² No medical contraindications for resistance training with blood flow restriction No regular physical activity in the past six months General health confirmed by a physician, including cardiovascular and blood pressure assessment No use of specific medications or supplements affecting sports performance or hormones

Exclusion criteria

Exclusion criteria: History of cardiovascular disease or uncontrolled hypertension Presence of chronic conditions such as diabetes, kidney, or liver failure Physical or musculoskeletal limitations preventing resistance training Use of medications affecting hormones or metabolism Consumption of sports supplements or anabolic steroids Recent injury or surgery affecting physical performance

Design outcomes

Primary

Measure	Time frame
Follistatin. Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Measured using the enzyme-linked immunosorbent assay (ELISA) method with kits from R&D Systems, USA.;IGF-1 levels. Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Measured using the ELISA method with kits from Mediagnost, Germany.;Body fat percentage. Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Measured using a bioelectrical impedance analysis device, Olympia 3.3, manufactured in South Korea.;Muscular strength (leg press and bench press). Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Assessed through the one-repetition maximum (1RM) test and calculated using the Brzycki formula.	—

Measure

Time frame

Follistatin. Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Measured using the enzyme-linked immunosorbent assay (ELISA) method with kits from R&D Systems, USA.;IGF-1 levels. Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Measured using the ELISA method with kits from Mediagnost, Germany.;Body fat percentage. Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Measured using a bioelectrical impedance analysis device, Olympia 3.3, manufactured in South Korea.;Muscular strength (leg press and bench press). Timepoint: It will be measured 48 hours before the start and 48 hours after the end of the intervention. Method of measurement: Assessed through the one-repetition maximum (1RM) test and calculated using the Brzycki formula.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactAkram Jafari

Islamic Azad University

jafari.akm@gmail.com0098383361001

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026