Effect of astaxanthin on the expression of factors involved in mitophagy and mitochondrial biogenesis in ovarian granulosa cells of women with Polycystic ovary syndrome who are candidates for ART

Effect of astaxanthin on the expression of factors involved in mitophagy and mitochondrial biogenesis in ovarian granulosa cells of women with Polycystic ovary syndrome who are candidates for ART: a three-blind randomized controlled clinical trial

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20231107059982N1

Enrollment

Registered

2025-02-21

Start date

2023-12-23

Completion date

Unknown

Last updated

2025-07-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Polycystic ovary syndrome. Polycystic ovarian syndrome

Interventions

Intervention 1: Intervention group: daily intake of 12 mg of astaxanthin for 25 eligible patients with polycystic ovary syndrome for six weeks. Intervention 2: Control group: daily use of placebo for

Eligibility

Sex/Gender

Female

Age

18 Years to 40 Years

Inclusion criteria

Inclusion criteria: Body mass index (BMI) between 20 and 30 PCOS diagnosis based on the Rotterdam criteria and having at least two of the three criteria

Exclusion criteria

Exclusion criteria: pregnancy Adrenal hyperplasia Androgen secreting tumors diabetes Hyperprolactinemia Thyroid dysfunction Infertility caused by male disorders Those who have taken antioxidant supplements, ovulation stimulating drugs, or drugs affecting the hormonal profile six months before starting the plan

Design outcomes

Primary

Measure	Time frame
Evaluation of expression of mitochondrial biogenesis pathway genes including Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a), Sirtuin 1 (SIRT1) and Mitochondrial transcription factor A (TFAM) in granulosa cells. Timepoint: End of study (end of intervention). Method of measurement: Real time pcr method.;Evaluation of the expression of PTEN-induced putative protein kinase 1 (PINK1) and Parkin proteins in granulosa cells. Timepoint: End of study (end of intervention). Method of measurement: Western blot method.;Levels of total antioxidant capacity (TAC) and Malondialdehyde (MDA) in follicular fluid. Timepoint: End of study (end of intervention). Method of measurement: ELISA method.;Outcomes of in vitro fertilization. Timepoint: End of study (end of intervention). Method of measurement: Microscopic examination.	—

Measure

Time frame

Evaluation of expression of mitochondrial biogenesis pathway genes including Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a), Sirtuin 1 (SIRT1) and Mitochondrial transcription factor A (TFAM) in granulosa cells. Timepoint: End of study (end of intervention). Method of measurement: Real time pcr method.;Evaluation of the expression of PTEN-induced putative protein kinase 1 (PINK1) and Parkin proteins in granulosa cells. Timepoint: End of study (end of intervention). Method of measurement: Western blot method.;Levels of total antioxidant capacity (TAC) and Malondialdehyde (MDA) in follicular fluid. Timepoint: End of study (end of intervention). Method of measurement: ELISA method.;Outcomes of in vitro fertilization. Timepoint: End of study (end of intervention). Method of measurement: Microscopic examination.

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactMahsa Motamed

Tehran University of Medical Sciences

Mahsa.motamed69@yahoo.com+98 21 6640 5357

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026