Overweight. Overweight
Conditions
Interventions
Intervention 1: Intervention group: Before the start of 8 weeks of training, the subjects of the intervention groups of speed exercise snacks training along with caloric restriction and the interventi
Sponsors
The University of Shahid Behshti
Eligibility
Sex/Gender
Male
Age
18 Years to 35 Years
Inclusion criteria
Inclusion criteria: healthy men. Body mass index 25-30 kg/m2. Not participating in regular exercise training during the past 6 months before the implementation of the research project. Not taking antibiotics in the last 6 months
Exclusion criteria
Exclusion criteria: History of taking illegal drugs and substances History of specific diseases such as alcoholic or non-alcoholic fatty liver, gallstones, diabetes, cardiovascular failure, metabolic syndrome, gastrointestinal syndrome
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Bile acids, the final product of cholesterol catabolism, are made by liver cells and stored in the gallbladder. In this research, selected species are included, primary bile acid i.e. chenodeoxycholic acid(CDCA), secondary bile acid i.e. lithocholic acid(LCA), bile acid conjugated with glycine i.e. glycocondeoxycholic acid(GCDCA), bile acid conjugated with taurine i.e. taurosodeoxycholic acid(TUDCA), total bile acids(TBA). Timepoint: before and after 8 weeks of intervention and control. Method of measurement: Liquid chromatography-mass spectrometry (LC-MS/MS) and Enzymatic assays. | — |
Secondary
| Measure | Time frame |
|---|---|
| Fibroblastic growth factor 19 regulates the production of bile acids. Timepoint: before and after 8 weeks of intervention and control. Method of measurement: By ELISA method.;7-alpha hydroxycholesten-3-1 is a confirmed indicator of CYP7A1 enzyme activity of cholesterol 7 alpha hydroxylase (an enzyme that leads to the production of bile acids from cholesterol). Timepoint: before and after 8 weeks of intervention and control. Method of measurement: Liquid chromatography-mass spectrometry (LC-MS/MS).;The maximum effect of insulin is defined by the response and its necessary concentration to create half of the maximum effect, is insulin sensitivity . Timepoint: before and after 8 weeks of intervention and control. Method of measurement: By evaluating the homeostasis model (HOMA-IR), insulin sensitivity will be obtained using serum glucose and insulin concentrations.;They are one of the most abundant filamicrobiomes in the human intestine . Timepoint: before and after 8 weeks of intervention and control. Method of measurement: In this research, an extraction kit will be used to extract genomic DNA from stool samples. Finally, the V4 region of the bacterial 16S rRNA genes will be subjected to PCR amplification to determine the characteristics of the microbiota. The community composition of each sample and the abundance of OTUs at the levels of phylum, class, order, family, genus and species will be counted. Finally, the ratio of Firmicutes/Bactoroidetes will be determined. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactHamid Irandoost
The University of Shahid Behshti
Outcome results
None listed