The Effect of High-Intensity Interval Training and Intermittent Fasting on Body Composition and Cellular Health

The effects of high-intensity interval training (HIIT) and intermittent fasting (IF) on body composition, oxidative stress, mitochondrial function, and autophagy: A randomized controlled trial

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20230804059029N2

Enrollment

188

Registered

2025-05-27

Start date

2025-05-31

Completion date

Unknown

Last updated

2025-07-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Prevention of metabolic disorders and cellular dysfunction in adults. Encounter for screening for other specified diseases and disorders

Interventions

Intervention 1: Intervention Group 1: The High-Intensity Interval Training (HIIT) group will follow a standard HIIT protocol consisting of 30-second exercise bouts performed at 85–90% of maximum heart

Eligibility

Sex/Gender

All

Age

20 Years to 45 Years

Inclusion criteria

Inclusion criteria: Aged between 20 and 45 years BMI ranging from 22 to 30 no history of chronic metabolic, cardiovascular, or nutritional diseases no engagement in regular intense exercise in the past six months and no use of medications affecting metabolism

Exclusion criteria

Exclusion criteria: Presence of chronic or metabolic diseases History of intense exercise Use of specific supplements or medications

Design outcomes

Primary

Measure	Time frame
In this study, the primary outcome variables include body composition (fat mass, lean mass, and bone mineral density), oxidative stress markers (such as MDA and total antioxidant capacity), gene and protein expression of key metabolic signaling pathways (including AMPK and mTOR), and cellular autophagy levels (measured via LC3 and Beclin-1 expression). These variables are selected to comprehensively assess the physiological and cellular effects of the exercise and dietary interventions. Timepoint: Outcome variables will be measured at three time points: before the intervention, at the end of week 12, and at the end of week 24. Method of measurement: Body composition will be assessed using DEXA (Dual-Energy X-ray Absorptiometry) scanning. Oxidative stress markers will be analyzed from blood samples using standard biochemical methods. Gene expression related to signaling pathways and autophagy will be measured through RNA extraction from peripheral cells, followed by RT-PCR and Western blot techniques.	—

Measure

Time frame

In this study, the primary outcome variables include body composition (fat mass, lean mass, and bone mineral density), oxidative stress markers (such as MDA and total antioxidant capacity), gene and protein expression of key metabolic signaling pathways (including AMPK and mTOR), and cellular autophagy levels (measured via LC3 and Beclin-1 expression). These variables are selected to comprehensively assess the physiological and cellular effects of the exercise and dietary interventions. Timepoint: Outcome variables will be measured at three time points: before the intervention, at the end of week 12, and at the end of week 24. Method of measurement: Body composition will be assessed using DEXA (Dual-Energy X-ray Absorptiometry) scanning. Oxidative stress markers will be analyzed from blood samples using standard biochemical methods. Gene expression related to signaling pathways and autophagy will be measured through RNA extraction from peripheral cells, followed by RT-PCR and Western blot techniques.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactBehnam Bagherzadeh-Rahmani

Hakim Sabzevari University

b.bagherzadehrahmani@gmail.com+98 11 4308 2713

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026