Quercetin in of oligoasthenoteratozoospermia

The effect of quercetin on microRNA-27a and microRNA-144 of the Nrf2 pathway, oxidative stress indices and sperm parameters in semen sample of oligoasthenoteratozoospermia patient candidate for ART

Status

Recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20230621058550N1

Enrollment

Registered

2023-08-31

Start date

2023-09-23

Completion date

Unknown

Last updated

2023-10-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

oligoasthenoteratozoospermia. Oligoasthenoteratozoospermia

Interventions

Intervention 1: Intervention group: Intervention group: Quercetin consumption. In this group, after Semen analysis and confirming the disease of oligoasthenoteratozoospermia, the patients receive the

Eligibility

Sex/Gender

Male

Age

25 Years to 40 Years

Inclusion criteria

Inclusion criteria: patients with oligoasthenoteratozoospermia induced infertility the age of 25-40 years BMI between 18 and 25

Exclusion criteria

Exclusion criteria: genetic and chromosomal disorders syndromes varicocele cryptorchidism orchitis and trauma history of chemotherapy radiation therapy or trauma to the testicles Smokers female infertility factors.

Design outcomes

Primary

Measure	Time frame
Nrf2 gene. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Real time pcr.;MicroRNA-27a. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Real time pcr.;MicroRNA-144. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Real time pcr.;DNA fragmentation. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Tunel kit.	—

Measure

Time frame

Nrf2 gene. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Real time pcr.;MicroRNA-27a. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Real time pcr.;MicroRNA-144. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Real time pcr.;DNA fragmentation. Timepoint: before and 75 days after the beginning of the intervention. Method of measurement: Tunel kit.

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Secondary

Measure	Time frame
Sperm motility. Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Counting,light microscope.;Sperm morphology. Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Diff_quick, light microscope.;Number and quality of embryos. Timepoint: 75-78 days after the beginning of the intervention. Method of measurement: Counting,light microscope.;Chemical pregnancy. Timepoint: 14 days after embryo transfer. Method of measurement: beta-hCG blood test Description.;Clinical pregnancy. Timepoint: five weeks after embryo transfer. Method of measurement: Observation by ultrasound.;Malondialdehyde (MDA). Timepoint: Before and 75 days after the intervention. Method of measurement: Spectrophotometry.;Total antioxidant capacity (TAC). Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Spectrophotometry.;Superoxide dismutase (SOD). Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Spectrophotometry.;Sperm concentration. Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Counting,light microscope.	—

Measure

Time frame

Sperm motility. Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Counting,light microscope.;Sperm morphology. Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Diff_quick, light microscope.;Number and quality of embryos. Timepoint: 75-78 days after the beginning of the intervention. Method of measurement: Counting,light microscope.;Chemical pregnancy. Timepoint: 14 days after embryo transfer. Method of measurement: beta-hCG blood test Description.;Clinical pregnancy. Timepoint: five weeks after embryo transfer. Method of measurement: Observation by ultrasound.;Malondialdehyde (MDA). Timepoint: Before and 75 days after the intervention. Method of measurement: Spectrophotometry.;Total antioxidant capacity (TAC). Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Spectrophotometry.;Superoxide dismutase (SOD). Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Spectrophotometry.;Sperm concentration. Timepoint: Before and 75 days after the intervention Method of measurement. Method of measurement: Counting,light microscope.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactDr Mahdi Abbasi

Tehran University of Medical Sciences

abbasima@sina.tums.ac.ir+98 21 6641 9072

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026