Effect of seminal exosome on the quality of frozen sperm

Evaluation of protective effect of seminal exosomes on the quality of Frozen-Thawed Sperms in oligoasthenoteratozoospermia men

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20230207057345N1

Enrollment

100

Registered

2023-05-21

Start date

2023-05-22

Completion date

Unknown

Last updated

2023-05-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Oligoasthenoteratozoospermia.

Interventions

Intervention 1: Intervention group 1: sperm of normozoospermic men incubate with exosomes extracted from men with oligoasthenoteratozoospermia (OAT). Intervention 2: Intervention group 2: sperm of OAT

Eligibility

Sex/Gender

Male

Age

25 Years to 40 Years

Inclusion criteria

Inclusion criteria: Normozoospermia men Oligoasthenoteratozoospermia men age of 25-40 years

Exclusion criteria

Exclusion criteria: Smokers varicocele genital infections hydrocele endocrine diseases such as diabetes

Design outcomes

Primary

Measure	Time frame
Sperm motility. Timepoint: after sperm thawing. Method of measurement: CASA software.;Total anti-oxidant capacity. Timepoint: after thawing. Method of measurement: Elisa kit.;Mitochondrial membrane potential. Timepoint: after thawing. Method of measurement: flow cytometry.;DNA breakage. Timepoint: after thawing. Method of measurement: flow cytometry.;Apoptosis. Timepoint: after thawing. Method of measurement: flow cytometry.;Sperm count. Timepoint: after thawing. Method of measurement: neubauer lam.	—

Measure

Time frame

Sperm motility. Timepoint: after sperm thawing. Method of measurement: CASA software.;Total anti-oxidant capacity. Timepoint: after thawing. Method of measurement: Elisa kit.;Mitochondrial membrane potential. Timepoint: after thawing. Method of measurement: flow cytometry.;DNA breakage. Timepoint: after thawing. Method of measurement: flow cytometry.;Apoptosis. Timepoint: after thawing. Method of measurement: flow cytometry.;Sperm count. Timepoint: after thawing. Method of measurement: neubauer lam.

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactFardin Amidi

Tehran University of Medical Sciences

amidifardin@yahoo.com+98 21 6405 3411

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026