effect of ß-Hydroxybutyrate and HIIT on gene expressions of Klotho/FGF axis an inflammation

Effect of acute ß-Hydroxybutyrate supplementation in combination with high-intensity interval training on gene expressions of Klotho/FGF axis in PBMC and inflammatory factors in inactive healthy adults.

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20221106056419N1

Enrollment

Registered

2023-01-05

Start date

2023-01-01

Completion date

Unknown

Last updated

2025-08-19

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

beta hydroxybutyrate, ketone body, Klotho, FGF23, FGF21, HIIT, high intensity interval, aging.

Interventions

Intervention group1: high intensity interval training(HIIT) includes 27 minutes of exercise on a stationary bike, including 6 cycles, each cycle consists of 4.5 minutes, with 4 minutes of recovery at

Eligibility

Sex/Gender

All

Age

18 Years to 30 Years

Inclusion criteria

Inclusion criteria: BMI between 18.5 to 30 not to have regular physical activity more than 1h and 3 days a week. not to lose or gain weight more than 5kg in the last 3 months. not to pregnant not to have breastfeeding

Exclusion criteria

Exclusion criteria: to have chronic and acute inflammatory and infectious diseases to have high blood pressure to have cardiovascular diseases to have diabetes or use diabetes medication alcohol consumption use thyroid medication

Design outcomes

Primary

Measure	Time frame
The effect of HIIT and beta-hydroxybutyrate intervention on Klotho gene expression in Peripheral Blood Mononuclear Cells(PBMC). Timepoint: Determination of Klotho gene expression level in Peripheral Blood Mononuclear Cells (PBMC) at the beginning of the intervention and 45 minutes after the intervention. Method of measurement: real time PCR.	—

Secondary

Measure	Time frame
1. Determining the effect of HIIT and beta-hydroxybutyrate intervention on FGF23 gene expression in peripheral blood mononuclear cells. 2.Determining the effect of HIIT and beta-hydroxybutyrate intervention on FGF21 gene expression in peripheral blood mononuclear cells. 3.Determining the effect of HIIT and beta-hydroxybutyrate intervention on hsCRP plasma level. Timepoint: Examination of all outcomes at the beginning of the intervention and 45 minutes after the intervention. Method of measurement: Real-time PCR method is used to determine FGF23 and FGF21 gene expression, and biochemical kit is used to determine hsCRP plasma level.	—

Measure

Time frame

1. Determining the effect of HIIT and beta-hydroxybutyrate intervention on FGF23 gene expression in peripheral blood mononuclear cells. 2.Determining the effect of HIIT and beta-hydroxybutyrate intervention on FGF21 gene expression in peripheral blood mononuclear cells. 3.Determining the effect of HIIT and beta-hydroxybutyrate intervention on hsCRP plasma level. Timepoint: Examination of all outcomes at the beginning of the intervention and 45 minutes after the intervention. Method of measurement: Real-time PCR method is used to determine FGF23 and FGF21 gene expression, and biochemical kit is used to determine hsCRP plasma level.

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Countries

Iran (Islamic Republic of)

Contacts

Public Contactdr. Arman Ghorbani

Shahid Beheshti University of Medical Sciences

ghorbani.nut@gmail.com+98 21 2235 7483

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026