Participants are healthy individuals..
Conditions
Interventions
Sponsors
Eligibility
Inclusion criteria
Inclusion criteria: Male semi-endurance athletes Age range 18 to 30 years No use of any supplements or drugs that affect the central nervous system Oxygen consumption greater than 55 milliliters per kilogram of body weight per minute (mL/Kg.min-1)
Exclusion criteria
Exclusion criteria: Unwillingness to continue cooperation at any stage of the research Injury during the research Failure to attend any of the assessment sessions History of seizures, epilepsy or other types of neurological diseases Use of tobacco, drugs, alcohol and caffeine supplements before the start of the intervention until the end of the intervention
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Tryptophan. Timepoint: Before the intervention and immediately after the subjects declare their inability to continue the exercise test, 2 cc of blood will be taken from their brachial vein to assess serum levels of tryptophan. Method of measurement: To assess serum tryptophan levels, commercial kits from the German company Zellbio will be used using the ELISA method.;Kynurenine. Timepoint: Before the intervention and immediately after the subjects declare their inability to continue the exercise test, 2 cc of blood will be taken from their brachial vein to assess serum kynurenine levels. Method of measurement: To assess serum kynurenine levels, commercial kits from the German company Zellbio will be used using the ELISA method.;Branched-chain amino acids. Timepoint: Before the intervention and immediately after the subjects declare their inability to continue the exercise test, 2 cc of blood will be taken from their brachial vein to assess serum branched chain amino acids levels. Method of measurement: To assess serum branched-chain amino acids levels, commercial kits from the German company Zellbio will be used using the ELISA method.;Free fatty acids. Timepoint: Before the intervention and immediately after the subjects declare their inability to continue the exercise test, 2 cc of blood will be taken from their brachial vein to assess serum free fatty acids levels. Method of measurement: Free fatty acids will also be measured by colorimetric method with a highly sensitive kit with a sensitivity of 5 µmol/L, Zellbio brand, made in Germany. | — |
Secondary
| Measure | Time frame |
|---|---|
| Time to exhaustion. Timepoint: The time point of assessment is immediately after the athlete reaches exhaustion. Method of measurement: By Bruce Treadmill Test.;Maximum oxygen consumption. Timepoint: The time point of assessment is immediately after the athlete reaches exhaustion. Method of measurement: By Bruce Treadmill Test. | — |
Countries
Iran (Islamic Republic of)
Contacts
The University of Kurdistan