The Effect of Aerobic Training with Vitamin D Supplementation on Cardiomyocytes Gene Expression of Type 2 Diabetic Rats.

The Effect of Eight Weeks Aerobic Training with Vitamin D Supplementation on Cardiac Biomarkers, VEGF-B Protein Levels, IGF-1 and Mir-1 Gene Expression in Cardiomyocytes of Type 2 Diabetic Rats.

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20220512054832N1

Enrollment

Registered

2022-06-09

Start date

2022-07-21

Completion date

Unknown

Last updated

2022-06-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

type 2 diabetes. Type 2 diabetes mellitus

Interventions

Intervention 1: Intervention group one (Aerobic training, type 2 diabetic models): Aerobic training for eight weeks (50-60% of maximum oxygen consumption for 25-60 minutes, five times per week). Inter

Receiving vitamin D for eight weeks (5000 IU per week). Intervention 3: Intervention group three (Vitamin D supplementation, type 2 diabetic models): Receiving vitamin D for eight weeks (5000 IU per w

Receiving vitamin D for eight weeks (5000 IU per week). Intervention 6: Intervention group six (Vitamin D supplementation, non-diabetic obese models): Receiving vitamin D for eight weeks (5000 IU per

Eligibility

Sex/Gender

Male

Age

2 Months to 3 Months

Inclusion criteria

Inclusion criteria: Age between 2 to 3 months old To have a minimum of 300 gram of weight To have type 2 diabetes in diabetic groups No to have type 2 diabetes in non-diabetic obese groups Male sex

Exclusion criteria

Exclusion criteria: Movement disabilities

Design outcomes

Primary

Measure	Time frame
Cardiac biomarkers. Timepoint: Before & after the intervention. Method of measurement: Elisa kit (Elabscience Company) with a sensitivity of 18,75 Pg/mL.;Vascular Endothelial Growth Factor B. Timepoint: Before & after the intervention. Method of measurement: Elisa kit (BT-LAB Company) with a sensitivity of 5.01 ng/L.;MicroRNA 1. Timepoint: Before & after intervention. Method of measurement: Polymerase chain reaction (PCR) method using The Corbett Research Rotor-Gene 6000 (QIAGEN company) and The miScript SYBR Green PCR Kit (QIAGEN company) with a sensitivity of 1.55 ng/ml.;Insulin-like growth factor 1. Timepoint: Before & after the intervention. Method of measurement: Polymerase chain reaction (PCR) method using The Corbett Research Rotor-Gene 6000 (QIAGEN company) and The Transcriptor First Strand cDNA Synthesis Kit (Roche company) with a sensitivity of 1.55 ng/ml.	—

Measure

Time frame

Cardiac biomarkers. Timepoint: Before & after the intervention. Method of measurement: Elisa kit (Elabscience Company) with a sensitivity of 18,75 Pg/mL.;Vascular Endothelial Growth Factor B. Timepoint: Before & after the intervention. Method of measurement: Elisa kit (BT-LAB Company) with a sensitivity of 5.01 ng/L.;MicroRNA 1. Timepoint: Before & after intervention. Method of measurement: Polymerase chain reaction (PCR) method using The Corbett Research Rotor-Gene 6000 (QIAGEN company) and The miScript SYBR Green PCR Kit (QIAGEN company) with a sensitivity of 1.55 ng/ml.;Insulin-like growth factor 1. Timepoint: Before & after the intervention. Method of measurement: Polymerase chain reaction (PCR) method using The Corbett Research Rotor-Gene 6000 (QIAGEN company) and The Transcriptor First Strand cDNA Synthesis Kit (Roche company) with a sensitivity of 1.55 ng/ml.

—

Secondary

Measure	Time frame
Lipid profile. Timepoint: Before & after the intervention. Method of measurement: Photometry method using Pars Azmoun company kits. The sensitivity of serum level assay kit for LDL Cholesterol, HDL Cholesterol, Triglyceride, and Glucose equals 12.098 mg/dL, 22.714 mg/dL, 35.087 mg/dL, and 25.497 mg/dL, respectively.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactRastegar Hosseini

Razi University of Kermanshah

rastegar.hoseini@gmail.com+98 83 3427 7605

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026