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The effect of the herring gum on the salivary bacteria, the salivary acidity, gingival plaque and bleeding

Clinical evaluation of the effect of Hypericum perforatum gum on salivary streptococcus mutans count, salivary pH, plaque index and gingival bleeding

Status
Recruiting
Phases
Unknown
Study type
Interventional
Source
IRCT
Registry ID
IRCT20211212053373N1
Enrollment
54
Registered
2022-01-23
Start date
2022-01-19
Completion date
Unknown
Last updated
2022-02-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Periodontal diseases. Periodontal disease, unspecified

Interventions

Intervention 1: Intervention group: The plant will be collected from the botanical garden of Tabriz University. After getting the approval of the herbarium of the University of Medical Sciences and ob

Sponsors

Tabriz University of Medical Sciences
Lead Sponsor

Eligibility

Sex/Gender
All
Age
18 Years to 65 Years

Inclusion criteria

Inclusion criteria: Participants should not have systemic disease Participants should have acceptable oral hygiene (they should not have obvious dental plaque) Age range: 18 to 65 years

Exclusion criteria

Exclusion criteria: Taking antibiotics or drugs that affect saliva Use of oral appliance Lack of proper cooperation People who breastfeed or are pregnant.

Design outcomes

Primary

MeasureTime frame
Percentage change in plaque index and gingival bleeding before and after chewing gum. Timepoint: Measurements at the beginning of the study and after 14 days of chewing gums. Method of measurement: Bleeding index, using periodontal prop in four levels of the sulcus, will be measured slowly for 30 seconds before and after using the chewing gum in each intervention and control group. Dental Plaque Index (DPI) will be calculated using the O’Leary index.;Measurement of streptococcus mutans in saliva. Timepoint: Measurements at the beginning of the study and after 14 days of using chewing gum. Method of measurement: Samples will be sent to the microbiology laboratory to determine the number of Streptococcus mutans colonies, the data of the control group and the intervention group will be analyzed using the Analysis of Covariance (ANCOVA), first in each group separately and then jointly. Saliva collection of these individuals will be done by the unstimulated method and will be cultured in order to count the number of Streptococcus mutans in a specific medium of Mitis salivarius agar at 37 ° C.;Saliva pH difference before and after using the vegetable gum. Timepoint: On the day 15 of the experiment. Method of measurement: 1 ml of saliva will be applied to the pH meter electrode for four consecutive measurements after 0, 5, 10, 15, 20 minutes of using the chewing gum. The final salivary pH will be recorded as the average of the three measurements. Independent t-test and covariance test will be used after measuring the pH of saliva in the two groups of control and intervention, before and after using chewing gum, with an average of three times.

Countries

Iran (Islamic Republic of)

Contacts

Public ContactDr. Maryam Kooh Soltani

Tabriz University of Medical Sciences

mkoohsoltani@yahoo.com+98 41 3335 5965

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026