Unexplained repeated implantation failure (uRIF). Female infertility, unspecified
Conditions
Interventions
Intervention 1: Intervention group: In the intervention group, endometrial sampling is obtained twice by Pipelle [one in the follicular phase (during 8-11 days and the last in the luteal phase (during
Sponsors
Iran University of Medical Sciences
Eligibility
Sex/Gender
Female
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: Infertile women with unexplained repeated implantation failure (unknown definite causes of RIF) failed to conceive after three or more embryo transfer cycles using high-quality transferred embryos (at least one blastocyst ET cycle) Age of 20 to 40 years Body mass index (BMI) less than 25 kg/m2 Good response to stimulation of previous ovulation Having at least two embryos with good grade normal uterus results of hysterosalpingography (HSG) or hysteroscopy Participant consent was required to join in the study and to complete the consent form
Exclusion criteria
Exclusion criteria: The thickness of the endometrium on the day of Human chorionic gonadotropin hormone injection is less than 7 mm Women with congenital anomalies, intramural and subserosal myomas( larger than 5 cm) Submucosal myoma Women with endometrioma larger than or equal to 3 cm With adhesions With hydrosalpinx Have previous uterine or ovarian surgery Severe male factor infertility (sperm extraction from the testis, sperm freezing, sperm DNA fragmentation index equal to or above 16%) Patients with any specific medication If the number of available embryos is less than 2 in the current cycle Women with endometrial tuberculosis and those undergoing tuberculosis treatment Have a history of diabetes, thyroid disease, any endocrine, genetic, infection or autoimmune disorder Abnormal Pre-implantation Genetic Test (PGT) Results Any specific medication Failure to return the patient to prepare an endometrial sample Women with severe pain during obtaining of tissue samples or the possibility of infection
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluation of gene expression. Timepoint: In the endometrial sample obtained in the luteal phase during days 19-23. Method of measurement: Using PCR Array method and based on the copy number. | — |
Secondary
| Measure | Time frame |
|---|---|
| Clinical pregnancy. Timepoint: 5 weeks after embryo transfer. Method of measurement: Vaginal ultrasonography (observing the gestational sac and fetal heart rate).;Implantation. Timepoint: 5 weeks after embryo transfer. Method of measurement: The number of observed sacs divided by the number of transferred embryos.;Total dose of gonadotropin (IU). Timepoint: From ovarian stimulation to human chorionic gonadotropin (hCG) injection. Method of measurement: The number of international units (IU) received during the ovarian stimulation cycle.;Duration of ovarian stimulation (day). Timepoint: From the start of gonadotropin administration to human chorionic gonadotropin (hCG) injection. Method of measurement: According to the patient's monitoring ultrasound chart (number of days receiving the medicine).;Number of retrieved oocytes. Timepoint: On the day of oocyte pick-up. Method of measurement: The number of retrieved oocytes reported in the embryology chart.;Metaphase II oocytes. Timepoint: On the day of oocyte pick-up. Method of measurement: Based on the presence of the polar body reported by the embryologist.;Number of embryos. Timepoint: Three or five days after oocyte pick-up. Method of measurement: The number of cleavage embryos reported by the embryologist.;Embryo transfer cancelation. Timepoint: Three or five days after oocyte pick-up. Method of measurement: Participants with no embryo transfer.;Chemical pregnancy rate/Embryo transfer. Timepoint: 2 weeks after embryo transfer. Method of measurement: Beta human chorionic gonadotropin (beta hCG) titer in blood serum by ELISA method.;Blighted ovum/Embryo transfer. Timepoint: 5 weeks after embryo transfer. Method of measurement: Absence of fetus in gestational sac according to ultrasound report.;Ectopic pregnancy/Embryo transfer. Timepoint: 5 weeks after embryo transfer. Method of measurement: According to the ultrasound report, there is a gestational sac outside the uterus with a positive Beta hCG titer.;Miscarriage r | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMehrdad Bakhtiyari
Iran University of Medical Sciences
Outcome results
None listed