The impact of intradermal lymphocyte therapy and probiotic supplement on recurrent implantation failure

The impact of intradermal lymphocyte therapy and probiotic supplement on macrophage polarization in women with recurrent implantation failure, characterized by an elevated TH1 to TH2 ratio

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20201021049101N2

Enrollment

Registered

2024-09-26

Start date

2024-08-22

Completion date

Unknown

Last updated

2025-01-08

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Recurrent implantation failure.

Interventions

Intervention 1: Intervention group:Twenty-five patients with recurrent implantation failure, randomly selected, will undergo lymphocyte therapy and probiotic supplementation. According to the protocol

Eligibility

Sex/Gender

Female

Age

18 Years to 40 Years

Inclusion criteria

Inclusion criteria: Be Azari-speaking people. Experience at least three recurrent implantation failures following IVF/ICSI. Do not have any type of immunotherapy in their medical history.

Exclusion criteria

Exclusion criteria: Patients under 18 years old and over 41 years old Patients who themselves or their spouses have abnormal karyotype or genetic and chromosomal disorders. Patients with coagulation disorders Patients who have an underlying chronic disease and have to take special drugs. Patients whose HIV, HCV or HBV test results are positive. Patients who have a history of asthma and allergy to certain drugs. Patients with intrauterine anomalies.

Design outcomes

Primary

Measure	Time frame
Successful implantation and pregnancy. Timepoint: The time of examining the individual in terms of success in implantation and pregnancy 5 months and 1 week after the start of the intervention (3 months of lymphocyte therapy and the use of probiotics + 1 month after the embryo transfer process + 5 weeks after ultrasound examination to see the gestational sac). Method of measurement: Performing an ultrasound to check the presence or absence of a gestational sac.;Increased polarization of endometrial tissue macrophages from M1 (inflammatory) to M2 (anti-inflammatory). Timepoint: After lymphocyte therapy and antibiotic therapy and before embryo transfer (3 months after the start of the intervention from time 0). Method of measurement: Examining the relative expression of genes related to macrophage function by Realtime PCR technique.	—

Measure

Time frame

Successful implantation and pregnancy. Timepoint: The time of examining the individual in terms of success in implantation and pregnancy 5 months and 1 week after the start of the intervention (3 months of lymphocyte therapy and the use of probiotics + 1 month after the embryo transfer process + 5 weeks after ultrasound examination to see the gestational sac). Method of measurement: Performing an ultrasound to check the presence or absence of a gestational sac.;Increased polarization of endometrial tissue macrophages from M1 (inflammatory) to M2 (anti-inflammatory). Timepoint: After lymphocyte therapy and antibiotic therapy and before embryo transfer (3 months after the start of the intervention from time 0). Method of measurement: Examining the relative expression of genes related to macrophage function by Realtime PCR technique.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactMehri Ghafourian

Ahvaz University of Medical Sciences

ghafourianbm@gmail.com+98 61 3311 2532

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026