infertility. Male infertility
Conditions
Interventions
Intervention 1: Intervention group: intracytoplasmic sperm injection using the zonapellucida-bound sperm to immature oocyte. Intervention 2: Control group: conventional intracytoplasmic sperm injectio
Sponsors
Yazd University of Medical Sciences
Eligibility
Sex/Gender
All
Age
No minimum to 40 Years
Inclusion criteria
Inclusion criteria: Age less than 40 years old At least six MII oocytes and one metaphase I oocyte retrieved Spouse semen samples with sperm concentration more than 5*106 motile spermatozoa
Exclusion criteria
Exclusion criteria: Non ejaculated spermatozoa (testicular or epididymal sperm retrieval) (for example: Azoospermia and cryptoazoospermia) cases in which the sperm cells were not bound to the zona pellucida for any reason Women with polycystic ovary syndrome
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Rate of clinical pregnancy. Timepoint: Rate of clinical pregnancy is evaluated 6 weeks after assisted reproductive technique. Method of measurement: Clinical pregnancy is measured through the recording of heart beat by ultrasonography. | — |
Secondary
| Measure | Time frame |
|---|---|
| Fertilization rate. Timepoint: Sixteen to 20 hours after performing of assisted reproductive technique. Method of measurement: Appearance of pronucleus by binocular loop observation.;Embryo quality. Timepoint: Three days after performing of assisted reproductive technique. Method of measurement: Total number of normal blastomer and percent of fragmented blastomer in defined time points using Time-Laps system.;Rate of chemical pregnancy. Timepoint: Two weeks after performing of assisted reproductive technique. Method of measurement: Measurement of serum Beta-Human Chorionic Gonadotropin.;Time lapse parameters. Timepoint: After intracytoplasmic injection of sperm (time 0), the following time points are measured: Time of disappearance of pronucleus in zygote (tPNF), Zygote divides into two cells (T2), three cells (T3), four cells (T4), five cells (T5), six cells (T6), seven cells (T7) and eight cells (T8). Method of measurement: Analyzing and measurement using EmbryoViewerTM software.;Rate of implantation. Timepoint: The rate of implantation is assessed 1 week to 10 days after the assisted reproduction technique. Method of measurement: Rate of implantation is determined by observation of gestational sac through the vaginal sonography. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMohammad Ali Khalili
Yazd University of Medical Sciences
Outcome results
None listed