Male factor infertility, evaluation of the effect of GM-CSF.
Conditions
Interventions
Intervention 1: Intervention group: After washing, the sperms are incubated with medium containing 2 ng / ml GM-CSF for one hour, then they used for ICSI. Intervention 2: Control group: After washing
Sponsors
Iran University of Medical Sciences
Eligibility
Sex/Gender
Male
Inclusion criteria
Inclusion criteria: Men with semen parameters such as sperm count, motility and morphology, lower than WHO reference values Men with Obstructive Azoospermia
Exclusion criteria
Exclusion criteria: Women over the age of 35, endometriosis, Polycystic ovary syndrome, Recurrent miscarriage
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sperm motility. Timepoint: Assessments of sperm motility at the beginning of the study and after incubation in both intervention and control groups. Method of measurement: Optical microscope.;Mitochondrial membrane potential of sperm. Timepoint: Measurement of mitochondrial membrane potential after incubation in both intervention and control groups. Method of measurement: Jc- 1, Flow cytometry.;Sperm DNA fragmentation. Timepoint: Measurement of sperm DNA fragmentation after incubation in both intervention and control groups. Method of measurement: TUNEL method.;Fertilization Rate. Timepoint: 24 hours after ICSI procedure. Method of measurement: View of 2 pronucleus with invert microscope.;Quality of embryo. Timepoint: Three days post-oocyte retrieval. Method of measurement: Using a three-point scoring system including fragmentation rate, number of blastomere, shape and size. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMahnaz Ashrafi
Iran University of Medical Sciences
Outcome results
None listed