Asthma. Asthma
Conditions
Interventions
Intervention 1: Intervention group will receive one probiotic supplement capsule include 1 billion colony forming unit (CFU) from Zisttakhmir company called Lactocare every day after the meal for 60 d
Sponsors
Semnan University of Medical Sciences
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: All patients with asthma who have dry- or with slight sputum cough sign. All patients with asthma who have wheezing sign. All patients with asthma who have dyspnea sign. All patients with asthma who sometimes have the chest tightness or pain symptom.
Exclusion criteria
Exclusion criteria: The patients who have lung infectious diseases such as pneumonia. The patients who have coronary heart disease. The patients who have lung cancer. The patients who have primary immunodeficiency. The patients who have secondary munodeficiency such as HIV. The patients who smoke. The patients who take antibiotics.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gene expression changes of T-bet, GATA3, Ror?t and FoxP3 transcription factors in peripheral blood mononuclear cells. Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using real-time PCR method.;Frequency changes of Treg cells in peripheral blood. Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using a flow cytometry.;Gene expression changes of miR 21, miR 155, miR146a, miR 126, miR 16 and miR133b in serum. Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using real-time PCR method.;Changes in serum level of IL-4 and IFN-?. Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using ELISA method.;Gene expression changes of IL-6, IL-17, IL-21 and TGF-ß in peripheral blood mononuclear cells. Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using real-time PCR method.;Change rates of Forced Expiratory Volume in first second (FEV1). Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using Spirometery.;Change rates of Forced Vital Capacity (FVC). Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using Spirometery.;Change rates of Forced Expiratory Volume in first second (FEV1)/Forced Vital Capacity (FVC). Timepoint: Before and 60 days after beginning of intervention. Method of measurement: Using Spirometery. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactRasoul Baharlou
Semnan University of Medical Sciences
Outcome results
None listed