patients with class II division I malocclusion or bimaxillary protrusion.
Conditions
Interventions
Intervention 1: Intervention group:Mini-screws were placed between the roots of maxillary second premolar and first molar. Diode laser was irradiated with 980 nm wavelength and 100 mW output power in
Sponsors
Yazd University of Medical Sciences
Eligibility
Sex/Gender
All
Age
15 Years to 18 Years
Inclusion criteria
Inclusion criteria: Patients with bimaxillary dentoalveolar protrusion or class II division I malocclusion requiring extraction of maxillary first premolars and anterior retraction. Good oral hygiene Signing the informed consent forms by the patients or their parents
Exclusion criteria
Exclusion criteria: systemic disease history of extraction of other permanent teeth taking medications affecting the level of pro-inflammatory cytokines such as non-steroidal anti-inflammatory drugs and corticosteroids during the study period or in the past one month prior to the onset of treatment periodontal disease or radiographic evidence of bone loss pregnancy during the orthodontic treatment
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary stability of mini-screws. Timepoint: at four time points: T0 (1 h after mini-screw placement), T1 (1 week later), T2 (at 1 month) and T3 (at 3 months).PTV was measured at the above mentioned four time points and one hour after laser irradiation. Method of measurement: The primary stability of mini-screws was measured by Periotest M and reported as Periotest value (PTV). At each time point, samples were collected from the PMCF to assess the concentration of IL-1B and TGF-B1.;Inflammation around the mini-screws. Timepoint: at four time points: T0 (1 h after mini-screw placement), T1 (1 week later), T2 (at 1 month) and T3 (at 3 months).The samples were collected at the aforementioned four time points and one hour after laser irradiation and prior to the measurement of PTV. Method of measurement: PMCF samples were collected using high-purity filter papers used as paper strips to measure the concentration of IL-1ß and TGF-ß1.A cotton roll was used for isolation of the area and the mini-screw site was dried with air spray for 15 seconds. The paper strip was inserted into the gingival sulcus around the mini-screw and remained there for 60 s. If the Periostrip was contaminated with blood or saliva, sampling was repeated. Immediately after PMCF collection, the paper strips were transferred into sterile 1.5 mL microtubes containing 250 µL of phosphate buffered saline. Parafilm (Bemis; Neenah, Wisconsin, USA) was used to seal the tubes. The vials were stored at -20°C until collection of all samples and onset of experiment. The PMCF samples were used to measure the concentration of IL-1ß using a kit designed for this purpose (Diaclone SAS, Besancon, France) and TGF-ß1 using the respective kit (IBL International GMBH, Germany) by enzyme-linked immunosorbent assay according to the manufacturers’ instructions. | — |
Secondary
| Measure | Time frame |
|---|---|
| At each time point, samples were collected from the PMCF to assess the concentration of IL-1B and TGF-B1. Timepoint: at four time points: T0 (1 h after mini-screw placement), T1 (1 week later), T2 (at 1 month) and T3 (at 3 months). Method of measurement: The PMCF samples were used to measure the concentration of IL-1B using a kit designed for this purpose (Diaclone SAS, Besancon, France) and TGF-B1 using the respective kit (IBL International GMBH, Germany) by enzyme-linked immunosorbent assay according to the manufacturers’ instructions. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactSomaye Kordi
Yazd University of Medical Sciences
Outcome results
None listed