Evaluation of the effectiveness of antimicrobial peptides nisin, melittin, pexiganan, tilapia pisidin, and omiganan as surgical hand disinfectants

Comparison of the efficacy of antimicrobial peptide combined with alcohol with a reference alcohol solution in surgical hand disinfection: A randomized clinical trial with a crossover design

Status

Recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20190924044863N4

Enrollment

Registered

2025-07-08

Start date

2025-06-25

Completion date

Unknown

Last updated

2025-07-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Surgeon's hand disinfection.

Interventions

Intervention 1: Intervention group: Antimicrobial peptide nisin, melittin, pexiganan, tilapia picidin, and omiganan with 4 times the minimum inhibitory concentration (MIC) along with 50% 1-ol propane.

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: Minimum age 18 years Minimum age 18 years Short and clean nails

Exclusion criteria

Exclusion criteria: Antibiotic use within 10 days prior to study entry Wearing jewelry and ornaments on hands and wrists during the study Use of antimicrobial creams, lotions, and soaps on hands three days before the study

Design outcomes

Primary

Measure	Time frame
Evaluation of the immediate and delayed effects of both types of interventions (use of the reference disinfectant and the test product). Timepoint: Immediately after the intervention (immediate assessment)Three hours after the intervention (delayed assessment). Method of measurement: Counting bacterial colonies in blood agar medium in both types of interventions (using the reference disinfectant and the test product).	—

Measure

Time frame

Evaluation of the immediate and delayed effects of both types of interventions (use of the reference disinfectant and the test product). Timepoint: Immediately after the intervention (immediate assessment)Three hours after the intervention (delayed assessment). Method of measurement: Counting bacterial colonies in blood agar medium in both types of interventions (using the reference disinfectant and the test product).

—

Secondary

Measure	Time frame
Acceptability of the product under test. Timepoint: After completing the study. Method of measurement: Questionnaire.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactElham Khosravi

Mashhad University of Medical Sciences

elhamkhelham71@gmail.com+98 51 3845 3239

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026