Effect of synbiotic supplementation in patients with ankylosing spondylitis

Effect of synbiotic supplementation on regulatory T cells (Tregs) frequency and related factors and disease activity in patients with ankylosing spondylitis

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20190917044794N2

Enrollment

Registered

2021-11-10

Start date

2020-12-21

Completion date

Unknown

Last updated

2021-11-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Ankylosing spondylitis. Ankylosing spondylitis

Interventions

Intervention 1: Intervention group: 1 synbiotic capsule fami lact per day for 3 months from Zist Takhmir factory capsuls include: 10 9 CFU bacteria namely Lactobacilus casei, Lactobacilus Ramnosis, La

Eligibility

Sex/Gender

All

Age

20 Years to 60 Years

Inclusion criteria

Inclusion criteria: Willing to participate in study Age between 20-60 years Diagnosis of ankylosing spondylitis by a rheumatologist

Exclusion criteria

Exclusion criteria: Taking nutritional and antioxidant supplements one month prior to and during study Pregnancy and lactation History of other autoimmune diseases, diabetes mellitus, IBD Gastrointestinal infection and other chronic diseases Recurrence of the disease during the study

Design outcomes

Primary

Measure	Time frame
T regulatory cells frequency. Timepoint: Before and after intervention. Method of measurement: Flow cytometry.;FoxP3 expression. Timepoint: Before and after intervention. Method of measurement: Real-time PCR.;TGF-B and IL-10 genes expression. Timepoint: Before and after intervention. Method of measurement: Real-time PCR.;Serum IL-10 and TGF-B level. Timepoint: Before and after intervention. Method of measurement: ELISA kit.	—

Measure

Time frame

T regulatory cells frequency. Timepoint: Before and after intervention. Method of measurement: Flow cytometry.;FoxP3 expression. Timepoint: Before and after intervention. Method of measurement: Real-time PCR.;TGF-B and IL-10 genes expression. Timepoint: Before and after intervention. Method of measurement: Real-time PCR.;Serum IL-10 and TGF-B level. Timepoint: Before and after intervention. Method of measurement: ELISA kit.

—

Secondary

Measure	Time frame
MiRNAs expression. Timepoint: Before and after intervention. Method of measurement: Real-time PCR.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactAida Malek Mahdavi

Tabriz University of Medical Sciences

amalek@tbzmed.ac.ir+98 41 3337 3992

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026