MACS method sperm washing in couples with male factor infertility

Comparing The DGC-Annexin V MACS method and DGC sperm washing method on sperm parameters, embryo development and PLCZeta gene expression in couples with male factor infertility

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20190804044430N1

Enrollment

Registered

2019-09-29

Start date

2019-06-22

Completion date

Unknown

Last updated

2019-11-05

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infertility due to male factor,The effect of MACS method on DNA fragmentation index (DFI).

Interventions

Intervention 1: Intervention group : After washing the sperm by DGC method, sperm selection is done by MACS method. In this procedure, washed sperm according to their apoptotic situation are adhered

Eligibility

Sex/Gender

Male

Inclusion criteria

Inclusion criteria: Infertile Couples Men with semen parameters (count,motility, morphology) lower than WHO guideline.

Exclusion criteria

Exclusion criteria: Women with Polycystic ovary syndrome (PCO) Women with endometriosis

Design outcomes

Primary

Measure	Time frame
Sperm motility. Timepoint: Sperm motility is analyzed at the beginning of the study and after sperm washing by either DGC or MACS-DGC methods. Method of measurement: optical microscope.;Sperm count. Timepoint: Sperm count is analyzed at the beginning of the study and after sperm washing by either DGC or MACS-DGC methods. Method of measurement: optical microscope.;Sperm morphology. Timepoint: Sperm morphology is analyzed at the beginning of the study and after sperm washing by either DGC or MACS-DGC methods. Method of measurement: optical microscope.	—

Measure

Time frame

Sperm motility. Timepoint: Sperm motility is analyzed at the beginning of the study and after sperm washing by either DGC or MACS-DGC methods. Method of measurement: optical microscope.;Sperm count. Timepoint: Sperm count is analyzed at the beginning of the study and after sperm washing by either DGC or MACS-DGC methods. Method of measurement: optical microscope.;Sperm morphology. Timepoint: Sperm morphology is analyzed at the beginning of the study and after sperm washing by either DGC or MACS-DGC methods. Method of measurement: optical microscope.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactZahra Zandieh

Iran University of Medical Sciences

zandie.dvm@gmail.com+98 21 86701

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026