Fertilization failure.
Conditions
Interventions
Intervention 1: Intervention group: Fertilization failure patient Current semen liquid processing techniques (such as the DGC met
Sponsors
Iran University of Medical Sciences
Eligibility
Sex/Gender
Male
Age
18 Years to 35 Years
Inclusion criteria
Inclusion criteria: A woman aged between 18 and 35 years old Fertilization failure after at least one ICSI cycle 28> Female BMI> 20 Normal sperm parameters No smoking in couples Agonist ovulation stimulation protocol Eggs in M2 stage
Exclusion criteria
Exclusion criteria: Severe endometriosis Varicocele in men Severe PCO
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Fertilization failure, sperm preparation methods. Timepoint: Fertilization failure: Diagnosis of file patients before entering the study. Methods of sperm preparation: After sampling, semen is divided into two groups and washed with two methods of preparation.?. Method of measurement: Fertilization failure: Based on patient records, sperm preparation methods: DGC and microfluidics. | — |
Secondary
| Measure | Time frame |
|---|---|
| Gene expression changes in studied groups. Timepoint: At the end of the study, after freeze-thawed semen. Method of measurement: Real-time PCR.;Sperm parameters. Timepoint: after semen sampling (before preparation) and after both microfluidic and DGC preparation methods. Method of measurement: Semen Analysis( Using Meckler Lam and Light Microscope).;DFI sperm. Timepoint: semen sampling (before preparation) and after both microfluidic and DGC preparation methods. Method of measurement: Halo Method.;Fertilization rate (number of embryos obtained). Timepoint: 18 to 20 hours after ICSI. Method of measurement: Light Microscope.;Quality of obtained embryos. Timepoint: after 48 and 72 hours of ICSI. Method of measurement: Light microscope. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactFatemeh sadat amjadi
Iran University of Medical Sciences
Outcome results
None listed