Cesarean section surgery. Delivery by caesarean section, unspecified
Conditions
Interventions
Intervention 1: Intervention group: In this group, after the closure of the uterine myometrium incision, PRP will be directly applied to the uterine myometrium tissue whereas the control group receiv
(the recommended temperature during processing is 21?–24? to prevent platelet activation during centrifugation of the blood)
2. The blood will be centrifuged at 1,200 rpm for 12 minutes
3. The blood separates into three layers: an upper layer that contains platelets and white blood cells, an intermediate thin layer (the buffy coat) that is rich in white blood cells, and a bottom laye
4. The upper and intermediate buffy layers will be transferred to an empty sterile tube. The plasma will be centrifuged again at 3,300 rpm for 7 minutes to help with the formation of soft pellets (ery
5. The upper two-thirds of the plasma will be discarded because it is platelet-poor plasma.6. Pellets are homogenized in the lower third (5 mL) of the plasma to create the PRP
the PRP will be ready for injection. Approximately 30 mL of venous blood yields 3–5 mL of PRP.7. The prepared PRP solution will be transferred within sterile syringe from the laboratory to the OR 8. T
Sponsors
Iran University of Medical Sciences
Eligibility
Sex/Gender
Female
Inclusion criteria
Inclusion criteria: Emergency and non-emergency cesarean section for the first time
Exclusion criteria
Exclusion criteria: Presence of uterine anomalies with or without previous repair Presence of uterine myoma at the site of caesarean incision History of myomectomy at site of caesarean incision Presence of placenta previa and placenta accrete Prolonged rupture of membranes without chorioamnionitis criteria (consist of fever, uterine tenderness…) Rupture of membranes with chorioamnionitis criteria (consist of fever, uterine tenderness…)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Uterine closure time. Timepoint: During the surgery. Method of measurement: Chronometer.;Primary postpartum hemorrhage. Timepoint: During and the day after surgery. Method of measurement: Complete Blood Count(CBC).;Pain. Timepoint: The day, week and month after surgery. Method of measurement: VAS scale.;Infection rate. Timepoint: The day, week and month after surgery. Method of measurement: Thermometer. | — |
Secondary
| Measure | Time frame |
|---|---|
| Residual myometrial thickness. Timepoint: 3 months after the surgery. Method of measurement: Ultrasound examination will be performed 3 months after the operation to assess the integrity of scar by measuring the residual myometrial thickness and the depth of the possible scar defect.;The depth of cesarean section scar defect(thickness). Timepoint: 3 months after the surgery. Method of measurement: Ultrasound examination will be performed 3 months after the operation to assess the integrity of scar by measuring the residual myometrial thickness and the depth of the possible scar defect. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactShahla Mirgaloybayat
Iran University of Medical Sciences
Outcome results
None listed