The anesthesia effect on serum levels and gene expression of interleukins in elective cesarean section

Comparison of the effect of general and spinal anesthesia on serum level and gene expression of interleukin 4, 6, 10 and 17; TGF-ß and IFN-? in maternal and umbelical cord blood in elective cesarean section

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT20180304038943N1

Enrollment

Registered

2018-04-17

Start date

2018-03-17

Completion date

Unknown

Last updated

2019-01-14

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

The effect of anesthesia technique on the immune system.

Interventions

Intervention 1: Intervention group 1 (general anesthesia group): In this group, after controlling the vital signs and administration of 100% oxygen using a mask and a pulse oximeter, induction of gene

Eligibility

Sex/Gender

Female

Age

20 Years to 45 Years

Inclusion criteria

Inclusion criteria: Having reading and writing skills being at the age of 20 to 45 years single pregnancy term pregnancy without complications such as Corioamnionitis and preterm rupture of membrane

Exclusion criteria

Exclusion criteria: Unwanted pregnancy History of underlying illness including hypertension, diabetes, cardiovascular disease History of immunodeficiency diseases including HIV and hepatitis B mental disorders Inflammatory diseases History of drug abuse, smoking, Opium addiction

Design outcomes

Primary

Measure	Time frame
Serum level of interleukin 4 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-4 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 6 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-6 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 10 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-10 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 17 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-17 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin IFN-? in maternal blood before and after intervention. Timepoint: Measurement of serum level of IFN-? before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin TGF-ß in maternal blood before and after intervention. Timepoint: Measurement of serum level of TGF-ß before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 4 in the umbilical cord blood of the baby. Timepoint: Immediately after birth. Method of measurement: Elisa test, Real Time PCR method.;Serum level of interleukin 6 in the umbilical cord blood of the baby. Timepoint: Immediately after birth. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 10 in the umbilical cord blood of the	—

Measure

Time frame

Serum level of interleukin 4 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-4 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 6 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-6 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 10 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-10 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 17 in maternal blood before and after intervention. Timepoint: Measurement of serum level of IL-17 before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin IFN-? in maternal blood before and after intervention. Timepoint: Measurement of serum level of IFN-? before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin TGF-ß in maternal blood before and after intervention. Timepoint: Measurement of serum level of TGF-ß before study, after uterine incision, 2 hours after surgery, 24 hours after surgery. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 4 in the umbilical cord blood of the baby. Timepoint: Immediately after birth. Method of measurement: Elisa test, Real Time PCR method.;Serum level of interleukin 6 in the umbilical cord blood of the baby. Timepoint: Immediately after birth. Method of measurement: ELISA test, Real Time PCR method.;Serum level of interleukin 10 in the umbilical cord blood of the

—

Secondary

Measure	Time frame
Post operative pain. Timepoint: 6, 12 and 24 hours after surgery. Method of measurement: Visual Analog Scale (VAS).	—

Countries

Iran (Islamic Republic of)

Contacts

Public Contactmaryam hadavi

Rafsanjan University of Medical Sciences

hadavi1344@rums.ac.ir+98 34280038

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026