Multiple Sclerosis. Multiple sclerosis
Conditions
Interventions
Intervention 1: Intervention group: Participants in this group will listen for 20 minutes, in a seated and relaxed state, to an audio file consisting of two different simultaneous frequencies (400 Hz
Sponsors
Bagheiat-allah University of Medical Sciences
Eligibility
Sex/Gender
Male
Age
20 Years to 50 Years
Inclusion criteria
Inclusion criteria: MS Patient Group: Male gender Age between 20 and 50 years Definitive diagnosis of multiple sclerosis confirmed by a neurologist Disease duration between 6 months and 5 years No systemic diseases such as diabetes, cardiovascular conditions, or other autoimmune disorders No history of neurological or psychiatric disorders including epilepsy, chronic headaches, migraine, ADHD, bipolar disorder, or acute anxiety No history of head or neck surgeries No use of psychoactive medications No smoking or similar substance use at least 24 hours before the test No regular physical activity in the past month Healthy Control Group: Male gender Age between 20 and 50 years Matched with the MS group in terms of age, sex, and body mass index (BMI) No systemic diseases such as diabetes, cardiovascular conditions, or other autoimmune disorders No history of neurological or psychiatric disorders including epilepsy, chronic headaches, migraine, ADHD, bipolar disorder, or acute anxiety No history of head or neck surgeries No use of psychoactive medications No smoking or similar substance use at least 24 hours before the test No regular physical activity in the past month
Exclusion criteria
Exclusion criteria:
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes in salivary cortisol levels, heart rate variability (HRV), and EEG synchronization in response to the auditory intervention are measured as physiological indicators of stress at three time points (before, during, and after the intervention). Timepoint: Measurements will be taken at three time points: Before the stress test (Baseline) Immediately after the Trier Social Stress Test (Pre-intervention) After the 20-minute auditory intervention (Post-intervention). Method of measurement: Salivary cortisol levels will be measured using a competitive enzyme-linked immunosorbent assay kit for human salivary cortisol.Heart rate variability will be analyzed using a three-channel electrocardiograph device and custom signal processing scripts in MATLAB software.Brainwave synchronization will be assessed using an eight-channel electroencephalography recording system and processed with EEGLAB toolbox in MATLAB. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactVahid Shaygannejad
Esfahan University of Medical Sciences
Outcome results
None listed