To investigate the efficacy of mesenchymal stem cell conditioned media (MSCs-CM) on ameliorate maturation and developmental potential of immature human oocytes after artificial activation in the infertile female.

To investigate the efficacy of mesenchymal stem cell conditioned media (MSCs-CM) on modulate apoptotic and stress-related gene expression, ameliorate maturation and developmental potential of immature human oocytes after artificial activation in the infertile female.

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT2017101736840N1

Enrollment

100

Registered

2017-11-11

Start date

2015-04-01

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Female infertility. Female infertility

Interventions

Intervention 1: Intervention group 1:50 germinal vesicles will be used in a-minimal essential medium (a-MEM) and then randomly be into fresh (fIVM). Intervention 2: Intervention group 2: 50 germinal v

Treatment - Drugs

Intervention group 1:50 germinal vesicles will be used in a-minimal essential medium (a-MEM) and then randomly be into fresh (fIVM).

Intervention group 2: 50 germinal vesicles will be used in a-minimal essential medium (a-MEM) and then randomly be into vitrification (vIVM).

Control group 1: 50 germinal vesicles will be used in MSCs-CM derived human umbilical cord and then randomly be into fresh (fIVM).

Control group 2: 50 germinal vesicles will be used in MSCs-CM derived human umbilical cord and then randomly be into vitrification (vIVM).

Eligibility

Sex/Gender

Female

Age

No minimum to 35 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria: the age lower than 35 years old. Exclusion criteria: endometriosis; poly cystic ovarian syndrome; abnormal chromosome; low ovarian response; chronic anovulation and fewer than five oocytes on retrieval.

Exclusion criteria

Exclusion criteria:

Design outcomes

Primary

Measure	Time frame
Expression of the BCL2, BAX, SOD and HSP70 genes. Timepoint: after oocytes maturation. Method of measurement: by Quantitative RT-PCR.	—

Secondary

Measure	Time frame
Maturity of oocytes. Timepoint: 24 and 48 hours after insertion in the medium. Method of measurement: by stereo microscope.;Developmental competence. Timepoint: 24 and 48 hours after insertion in the medium. Method of measurement: Ionomycin.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactHakime Akbari

Kerman University of Medical Sciences

ghsuper@sums.ac.it+98 34 3132 5700

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026